##gff-version 3
##sequence-region BBa_S01004 1 1905
BBa_S01004	.	terminator	1777	1856	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01004	.	stem_loop	1788	1831	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01004	.	engineered_region	1777	1856	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01004	.	promoter	926	980	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_S01004	.	engineered_region	926	980	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S01004	.	promoter	945	950	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S01004	.	promoter	967	972	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S01004	.	start_codon	978	978	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S01004	.	non_covalent_binding_site	926	945	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S01004	.	engineered_region	1007	1768	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_S01004	.	point_mutation	1075	1075	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S01004	.	tag	1725	1762	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S01004	.	CDS	1007	1768	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S01004	.	stop_codon	1763	1768	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S01004	.	engineered_region	1007	1768	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S01004	.	terminator	877	917	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01004	.	polyA_site	904	917	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01004	.	engineered_region	877	917	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01004	.	stem_loop	884	903	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01004	.	stop_codon	910	910	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01004	.	terminator	1865	1905	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01004	.	polyA_site	1892	1905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01004	.	engineered_region	1865	1905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01004	.	stem_loop	1872	1891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01004	.	stop_codon	1898	1898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01004	.	terminator	789	868	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01004	.	stem_loop	800	843	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01004	.	engineered_region	789	868	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01004	.	engineered_region	19	780	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01004	.	stop_codon	775	780	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01004	.	CDS	19	780	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01004	.	sequence_alteration	260	260	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01004	.	engineered_region	19	780	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01004	.	tag	736	774	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01004	.	ribosome_entry_site	989	1000	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01004	.	conserved_region	993	996	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01004	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01004	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S01004
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacg
gcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagtt
catctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcc
cgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatct
tcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagct
gaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctat
atcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagc
tcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacca
gtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcact
ctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttta
cgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggag
ctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagg
gcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccac
cctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaag
tccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgagg
tgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggg
gcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaac
ttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacg
gccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatca
catggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaac
gacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttata