##gff-version 3
##sequence-region BBa_S01005 1 1903
BBa_S01005	.	ribosome_entry_site	987	998	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01005	.	conserved_region	991	994	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01005	.	engineered_region	1005	1766	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_S01005	.	stop_codon	1761	1766	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S01005	.	point_mutation	1073	1073	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S01005	.	tag	1723	1760	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S01005	.	CDS	1005	1766	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S01005	.	engineered_region	1005	1766	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S01005	.	terminator	1863	1903	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01005	.	polyA_site	1890	1903	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01005	.	stem_loop	1870	1889	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01005	.	engineered_region	1863	1903	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01005	.	stop_codon	1896	1896	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01005	.	terminator	789	868	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01005	.	stem_loop	800	843	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01005	.	engineered_region	789	868	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01005	.	promoter	926	978	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_S01005	.	start_codon	978	978	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_S01005	.	promoter	944	949	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_S01005	.	promoter	967	972	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_S01005	.	non_covalent_binding_site	926	945	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_S01005	.	terminator	877	917	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01005	.	polyA_site	904	917	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01005	.	stem_loop	884	903	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01005	.	engineered_region	877	917	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01005	.	stop_codon	910	910	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01005	.	terminator	1775	1854	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01005	.	stem_loop	1786	1829	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01005	.	engineered_region	1775	1854	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01005	.	engineered_region	19	780	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01005	.	tag	736	774	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01005	.	sequence_alteration	260	260	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01005	.	engineered_region	19	780	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01005	.	CDS	19	780	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01005	.	stop_codon	775	780	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01005	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01005	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S01005
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacg
gcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagtt
catctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcc
cgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatct
tcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagct
gaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctat
atcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagc
tcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacca
gtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcact
ctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttacc
gttagctttcgaattggctaaaaagtgttctactagagaaagaggagaaatactagatggtgagcaagggcgaggagct
gttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggc
gagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccc
tcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtc
cgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtg
aagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggc
acaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaactt
caagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggc
cccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcaca
tggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacga
cgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata