##gff-version 3
##sequence-region BBa_S01016 1 1568
BBa_S01016	.	terminator	1440	1519	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01016	.	engineered_region	1440	1519	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01016	.	stem_loop	1451	1494	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01016	.	ribosome_entry_site	771	782	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01016	.	conserved_region	775	778	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01016	.	CDS	789	1406	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S01016	.	CDS	789	1367	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S01016	.	engineered_tag	1407	1431	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S01016	.	engineered_region	789	1406	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S01016	.	tag	1368	1399	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S01016	.	engineered_region	1	762	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01016	.	stop_codon	757	762	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01016	.	CDS	1	762	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01016	.	engineered_region	1	762	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01016	.	tag	718	756	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01016	.	sequence_alteration	242	242	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01016	.	terminator	1528	1568	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01016	.	engineered_region	1528	1568	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01016	.	stop_codon	1561	1561	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01016	.	stem_loop	1535	1554	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01016	.	polyA_site	1555	1568	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_S01016
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaca
agttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaa
gctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatg
aagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggca
actacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaa
ggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag
aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagc
agaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaaga
ccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctg
tacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagaaagaggagaaatactagat
gactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaa
gtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcag
aatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgct
gaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgct
gtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacg
ctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttcc
ttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaa
cagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagt
gtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata