LOCUS       BBa_S01020              2366 bp    DNA     linear       25-Nov-2024
DEFINITION  Q04121.Q04510
ACCESSION   BBa_S01020
VERSION     BBa_S01020.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     misc_feature    1319..1335
                     /label=lac O1
     promoter        1336..1341
                     /label=-35
     misc_feature    1342..1358
                     /label=lac O1
     promoter        1359..1364
                     /label=-10
     misc_feature    1317..1370
                     /label=BBa_R0011
     promoter        1317..1370
                     /label=BBa_R0011
     misc_feature    1384..1387
                     /label=conserved
     RBS             1380..1391
                     /label=BBa_B0034
     CDS             1401..2108
                     /label=cI lambda
     misc_feature    2109..2141
                     /label=LVA
     misc_feature    2148..2172
                     /label=Help:Barcodes
     CDS             1398..2147
                     /label=BBa_C0051
     stem_loop       2192..2235
                     /label=stem_loop
     misc_feature    2181..2260
                     /label=BBa_B0010
     terminator      2181..2260
                     /label=BBa_B0010
     stem_loop       2276..2295
                     /label=T7 TE
     misc_feature    2302..2302
                     /label=stop
     polyA_site      2296..2309
                     /label=polya
     misc_feature    2269..2309
                     /label=BBa_B0012
     terminator      2269..2309
                     /label=BBa_B0012
     promoter        2355..2360
                     /label=-10
     promoter        2332..2337
                     /label=-35
     misc_feature    2342..2358
                     /label=OR1
     misc_feature    2318..2334
                     /label=OR2
     misc_feature    2318..2366
                     /label=BBa_R0051
     promoter        2318..2366
                     /label=BBa_R0051
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt
     1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga
     1381 aagaggagaa atactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg
     1441 acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg
     1501 aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca
     1561 tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg
     1621 aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc
     1681 agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt
     1741 tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca
     1801 aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa
     1861 caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg
     1921 ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac
     1981 tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc
     2041 catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga
     2101 cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct
     2161 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2221 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2281 accttcgggt gggcctttct gcgtttatat actagagtaa caccgtgcgt gttgactatt
     2341 ttacctctgg cggtgataat ggttgc
//