LOCUS       BBa_S01283               903 bp    DNA     linear       25-Nov-2024
DEFINITION  Intermediate part from assembly 236
ACCESSION   BBa_S01283
VERSION     BBa_S01283.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             23..639
                     /label=tetR
     misc_feature    640..673
                     /label=SsrA
     misc_feature    680..704
                     /label=Help:Barcodes
     CDS             20..679
                     /label=BBa_C0040
     stem_loop       724..767
                     /label=stem_loop
     misc_feature    713..792
                     /label=BBa_B0010
     terminator      713..792
                     /label=BBa_B0010
     stem_loop       808..827
                     /label=T7 TE
     misc_feature    834..834
                     /label=stop
     polyA_site      828..841
                     /label=polya
     misc_feature    801..841
                     /label=BBa_B0012
     terminator      801..841
                     /label=BBa_B0012
     misc_feature    850..868
                     /label=TetR 1
     misc_feature    850..903
                     /label=BBa_R0040
     promoter        869..874
                     /label=-35
     misc_feature    875..893
                     /label=TetR 2
     promoter        892..897
                     /label=-10
     promoter        850..903
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
       61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
      661 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat
      721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      841 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag
      901 cac
//