LOCUS       BBa_S01284              1517 bp    DNA     linear       25-Nov-2024
DEFINITION  Intermediate part from assembly 236
ACCESSION   BBa_S01284
VERSION     BBa_S01284.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    1109..1147
                     /label=LVA
     CDS             20..1147
                     /label=lacI-LVA
     misc_feature    20..1147
                     /label=BBa_C0012
     misc_feature    1148..1172
                     /label=Help:Barcodes
     CDS             20..1147
                     /label=BBa_C0012
     stem_loop       1192..1235
                     /label=stem_loop
     misc_feature    1181..1260
                     /label=BBa_B0010
     terminator      1181..1260
                     /label=BBa_B0010
     stem_loop       1276..1295
                     /label=T7 TE
     misc_feature    1302..1302
                     /label=stop
     polyA_site      1296..1309
                     /label=polya
     misc_feature    1269..1309
                     /label=BBa_B0012
     terminator      1269..1309
                     /label=BBa_B0012
     CDS             1318..1405
                     /label=end of LacI coding region (inactive)
     misc_feature    1318..1517
                     /label=BBa_R0010
     misc_feature    1406..1443
                     /label=CAP binding site
     promoter        1454..1459
                     /label=-35
     promoter        1478..1483
                     /label=-10
     misc_feature    1483..1517
                     /label=LacI binding site
     misc_feature    1490..1490
                     /label=start
     promoter        1318..1517
                     /label=BBa_R0010
ORIGIN
        1 tcacacagga aagtactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
       61 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      121 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      181 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      241 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      301 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      361 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      421 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      481 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      541 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      601 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      661 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      721 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      781 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      841 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      901 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
      961 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1021 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1081 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1141 ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga
     1201 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1261 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa
     1321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     1441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1501 gataacaatt tcacaca
//