LOCUS       BBa_S01294               982 bp    DNA     linear       22-Nov-2024
DEFINITION  Intermediate part from assembly 236
ACCESSION   BBa_S01294
VERSION     BBa_S01294.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..783
                     /label=CFP (LVA)
     misc_feature    740..777
                     /label=SsrA
     misc_feature    778..783
                     /label=2
     misc_feature    90..90
                     /label=A
     misc_feature    22..783
                     /label=BBa_E0022
     misc_feature    22..783
                     /label=BBa_E0022
     stem_loop       803..846
                     /label=stem_loop
     misc_feature    792..871
                     /label=BBa_B0010
     terminator      792..871
                     /label=BBa_B0010
     stem_loop       887..906
                     /label=T7 TE
     misc_feature    913..913
                     /label=stop
     polyA_site      907..920
                     /label=polya
     misc_feature    880..920
                     /label=BBa_B0012
     terminator      880..920
                     /label=BBa_B0012
     misc_feature    929..947
                     /label=TetR 1
     misc_feature    929..982
                     /label=BBa_R0040
     promoter        948..953
                     /label=-35
     misc_feature    954..972
                     /label=TetR 2
     promoter        971..976
                     /label=-10
     promoter        929..982
                     /label=BBa_R0040
ORIGIN
        1 attaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
       61 cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag
      121 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      181 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
      241 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      301 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      361 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      421 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
      481 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
      541 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      601 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      661 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      721 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa
      781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      901 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat
      961 cagtgataga gatactgagc ac
//