##gff-version 3
##sequence-region BBa_S01294 1 982
BBa_S01294	.	promoter	929	982	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01294	.	promoter	971	976	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01294	.	non_covalent_binding_site	929	947	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01294	.	promoter	948	953	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01294	.	non_covalent_binding_site	954	972	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01294	.	engineered_region	929	982	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01294	.	terminator	880	920	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01294	.	polyA_site	907	920	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01294	.	stem_loop	887	906	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01294	.	engineered_region	880	920	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01294	.	stop_codon	913	913	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01294	.	engineered_region	22	783	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_S01294	.	stop_codon	778	783	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S01294	.	tag	740	777	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S01294	.	point_mutation	90	90	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S01294	.	CDS	22	783	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S01294	.	engineered_region	22	783	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S01294	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01294	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01294	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01294	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01294	.	terminator	792	871	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01294	.	stem_loop	803	846	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01294	.	engineered_region	792	871	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S01294
attaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgg
acggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaa
gttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttc
agccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacca
tcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcga
gctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtc
tatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgc
agctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcac
ccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatc
actctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagat
tgacatccctatcagtgatagagatactgagcac