##gff-version 3
##sequence-region BBa_S01295 1 920
BBa_S01295	.	terminator	792	871	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01295	.	stem_loop	803	846	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01295	.	engineered_region	792	871	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01295	.	terminator	880	920	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01295	.	polyA_site	907	920	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01295	.	engineered_region	880	920	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01295	.	stem_loop	887	906	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01295	.	stop_codon	913	913	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01295	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01295	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01295	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01295	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01295	.	engineered_region	22	783	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01295	.	stop_codon	778	783	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01295	.	CDS	22	783	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01295	.	sequence_alteration	263	263	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01295	.	engineered_region	22	783	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01295	.	tag	739	777	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
>BBa_S01295
attaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgg
acggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaa
gttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttc
gcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacca
tcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcga
gctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtc
tatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgc
agctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcta
ccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatc
actctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata