LOCUS       BBa_S01296              2024 bp    DNA     linear       22-Dec-2024
DEFINITION  Intermediate part from assembly 236
ACCESSION   BBa_S01296
VERSION     BBa_S01296.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    22..24
                     /label=start
     misc_feature    805..810
                     /label=2
     misc_feature    772..804
                     /label=LVA
     CDS             22..771
                     /label=aiiA
     misc_feature    22..810
                     /label=BBa_C0060
     misc_feature    811..835
                     /label=Help:Barcodes
     CDS             22..810
                     /label=BBa_C0060
     stem_loop       855..898
                     /label=stem_loop
     misc_feature    844..923
                     /label=BBa_B0010
     terminator      844..923
                     /label=BBa_B0010
     stem_loop       939..958
                     /label=T7 TE
     misc_feature    965..965
                     /label=stop
     polyA_site      959..972
                     /label=polya
     misc_feature    932..972
                     /label=BBa_B0012
     terminator      932..972
                     /label=BBa_B0012
     misc_feature    981..999
                     /label=TetR 1
     misc_feature    981..1034
                     /label=BBa_R0040
     promoter        1000..1005
                     /label=-35
     misc_feature    1006..1024
                     /label=TetR 2
     promoter        1023..1028
                     /label=-10
     promoter        981..1034
                     /label=BBa_R0040
     RBS             1043..1057
                     /label=RBS-1\Strong
     RBS             1050..1054
                     /label=RBS
     misc_feature    1043..1057
                     /label=BBa_B0030
     RBS             1043..1057
                     /label=BBa_B0030
     CDS             1064..1825
                     /label=CFP (LVA)
     misc_feature    1782..1819
                     /label=SsrA
     misc_feature    1820..1825
                     /label=2
     misc_feature    1132..1132
                     /label=A
     misc_feature    1064..1825
                     /label=BBa_E0022
     misc_feature    1064..1825
                     /label=BBa_E0022
     stem_loop       1845..1888
                     /label=stem_loop
     misc_feature    1834..1913
                     /label=BBa_B0010
     terminator      1834..1913
                     /label=BBa_B0010
     stem_loop       1929..1948
                     /label=T7 TE
     misc_feature    1955..1955
                     /label=stop
     polyA_site      1949..1962
                     /label=polya
     misc_feature    1922..1962
                     /label=BBa_B0012
     terminator      1922..1962
                     /label=BBa_B0012
     misc_feature    1971..1989
                     /label=TetR 1
     misc_feature    1971..2024
                     /label=BBa_R0040
     promoter        1990..1995
                     /label=-35
     misc_feature    1996..2014
                     /label=TetR 2
     promoter        2013..2018
                     /label=-10
     promoter        1971..2024
                     /label=BBa_R0040
ORIGIN
        1 attaaagagg agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt
       61 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta
      121 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg
      181 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt
      241 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag
      301 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat
      361 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat
      421 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt
      481 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat
      541 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg
      601 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct
      661 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt
      721 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac
      781 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct atcagtgata
     1021 gagatactga gcactactag agattaaaga ggagaaatac tagatggtga gcaagggcga
     1081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg tgaacggcca
     1141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1261 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1501 catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     1561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1741 cgccgccggg atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga
     1801 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1861 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1921 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag
     1981 tgatagagat tgacatccct atcagtgata gagatactga gcac
//