LOCUS       BBa_S01298              1114 bp    DNA     linear       22-Dec-2024
DEFINITION  Intermediate part from assembly 236
ACCESSION   BBa_S01298
VERSION     BBa_S01298.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     RBS             106..120
                     /label=RBS-1\Strong
     RBS             113..117
                     /label=RBS
     misc_feature    106..120
                     /label=BBa_B0030
     RBS             106..120
                     /label=BBa_B0030
     misc_feature    706..737
                     /label=LVA
     CDS             127..705
                     /label=luxI
     misc_feature    127..744
                     /label=BBa_C0061
     misc_feature    745..769
                     /label=Help:Barcodes
     CDS             127..744
                     /label=BBa_C0061
     stem_loop       789..832
                     /label=stem_loop
     misc_feature    778..857
                     /label=BBa_B0010
     terminator      778..857
                     /label=BBa_B0010
     stem_loop       873..892
                     /label=T7 TE
     misc_feature    899..899
                     /label=stop
     polyA_site      893..906
                     /label=polya
     misc_feature    866..906
                     /label=BBa_B0012
     terminator      866..906
                     /label=BBa_B0012
     CDS             915..1002
                     /label=end of LacI coding region (inactive)
     misc_feature    915..1114
                     /label=BBa_R0010
     misc_feature    1003..1040
                     /label=CAP binding site
     promoter        1051..1056
                     /label=-35
     promoter        1075..1080
                     /label=-10
     misc_feature    1080..1114
                     /label=LacI binding site
     misc_feature    1087..1087
                     /label=start
     promoter        915..1114
                     /label=BBa_R0010
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagattaa agaggagaaa
      121 tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc ggaggagtat
      181 aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt
      241 gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata tatttatgct
      301 tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac aggtgattat
      361 atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa agatcctaat
      421 atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa taactctgct
      481 agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag tcaaggtatt
      541 acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg tattaaagtt
      601 ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa atcggttgta
      661 ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc aaacgacgaa
      721 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca
      781 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      841 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      901 tttatatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      961 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     1081 gtgtggaatt gtgagcggat aacaatttca caca
//