##gff-version 3
##sequence-region BBa_S01299 1 4578
BBa_S01299	.	ribosome_entry_site	3640	3654	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01299	.	engineered_region	3640	3654	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01299	.	ribosome_entry_site	3647	3651	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01299	.	ribosome_entry_site	3640	3654	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01299	.	promoter	955	1008	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01299	.	promoter	974	979	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01299	.	non_covalent_binding_site	980	998	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01299	.	engineered_region	955	1008	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01299	.	promoter	997	1002	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01299	.	non_covalent_binding_site	955	973	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01299	.	terminator	3344	3423	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01299	.	engineered_region	3344	3423	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01299	.	stem_loop	3355	3398	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01299	.	terminator	4450	4529	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01299	.	engineered_region	4450	4529	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01299	.	stem_loop	4461	4504	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01299	.	terminator	4538	4578	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01299	.	engineered_region	4538	4578	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01299	.	stop_codon	4571	4571	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01299	.	stem_loop	4545	4564	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01299	.	polyA_site	4565	4578	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01299	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S01299	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S01299	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S01299	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S01299	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S01299	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S01299	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S01299	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S01299	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S01299	.	promoter	3481	3631	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_S01299	.	non_covalent_binding_site	3610	3629	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S01299	.	engineered_region	3481	3631	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S01299	.	promoter	3569	3574	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S01299	.	non_covalent_binding_site	3481	3500	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S01299	.	promoter	3595	3602	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S01299	.	start_codon	3608	3608	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S01299	.	promoter	2334	2533	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01299	.	non_covalent_binding_site	2422	2459	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01299	.	promoter	2494	2499	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01299	.	engineered_region	2334	2533	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01299	.	CDS	2334	2421	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01299	.	non_covalent_binding_site	2499	2533	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01299	.	promoter	2470	2475	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01299	.	start_codon	2506	2506	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01299	.	ribosome_entry_site	2542	2554	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S01299	.	ribosome_entry_site	2548	2551	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S01299	.	ribosome_entry_site	2542	2554	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S01299	.	engineered_region	2542	2554	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S01299	.	CDS	3661	4416	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S01299	.	point_mutation	4152	4152	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S01299	.	CDS	3661	4410	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S01299	.	engineered_region	3661	4416	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S01299	.	engineered_tag	4417	4441	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S01299	.	point_mutation	3834	3834	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S01299	.	engineered_region	3661	3662	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S01299	.	CDS	125	784	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01299	.	engineered_tag	785	809	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01299	.	tag	745	778	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01299	.	CDS	128	744	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01299	.	CDS	1036	2163	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_S01299	.	engineered_tag	2164	2188	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01299	.	engineered_region	1036	2163	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01299	.	CDS	1036	2163	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01299	.	tag	2125	2163	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01299	.	ribosome_entry_site	106	118	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S01299	.	ribosome_entry_site	112	115	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S01299	.	ribosome_entry_site	106	118	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S01299	.	engineered_region	106	118	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S01299	.	terminator	2197	2276	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01299	.	engineered_region	2197	2276	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01299	.	stem_loop	2208	2251	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01299	.	CDS	2561	3310	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S01299	.	tag	3272	3304	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01299	.	CDS	2564	3271	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01299	.	engineered_tag	3311	3335	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S01299	.	terminator	3432	3472	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01299	.	engineered_region	3432	3472	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01299	.	stop_codon	3465	3465	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01299	.	stem_loop	3439	3458	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01299	.	polyA_site	3459	3472	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01299	.	terminator	2285	2325	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01299	.	engineered_region	2285	2325	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01299	.	stop_codon	2318	2318	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01299	.	stem_loop	2292	2311	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01299	.	polyA_site	2312	2325	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01299	.	terminator	906	946	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01299	.	engineered_region	906	946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01299	.	stop_codon	939	939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01299	.	stem_loop	913	932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01299	.	polyA_site	933	946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01299	.	terminator	818	897	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01299	.	engineered_region	818	897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01299	.	stem_loop	829	872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01299	.	ribosome_entry_site	1017	1029	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S01299	.	ribosome_entry_site	1023	1026	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S01299	.	ribosome_entry_site	1017	1029	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S01299	.	engineered_region	1017	1029	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_S01299
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaaca
gcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagca
gcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccat
actcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttac
taagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatt
agcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggt
tgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattat
tacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgg
attagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactga
tagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaa
agtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttc
ccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattac
attcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgc
acgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtaga
acgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactat
ccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgacc
agacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtca
ccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctc
actcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaa
tgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattac
cgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccg
ccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcc
aggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgc
ctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaac
gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgat
tcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctc
actcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacatactagagtcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgc
acgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatgggg
atggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaa
ttctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgca
gccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacc
tttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaag
gtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggc
tgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggt
caggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagtta
tcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgata
gtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagacctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaat
ttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatac
tagagattaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagc
ttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcg
atcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatg
atgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatt
tgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagt
ttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtt
tatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaa
taataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggat
atttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaa
accgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgata
gtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata