##gff-version 3
##sequence-region BBa_S01301 1 858
BBa_S01301	.	ribosome_entry_site	58	72	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01301	.	ribosome_entry_site	58	72	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01301	.	ribosome_entry_site	65	69	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01301	.	engineered_region	58	72	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01301	.	CDS	79	696	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S01301	.	CDS	79	657	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S01301	.	engineered_region	79	696	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S01301	.	tag	658	689	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S01301	.	engineered_tag	697	721	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S01301	.	terminator	818	858	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01301	.	polyA_site	845	858	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01301	.	engineered_region	818	858	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01301	.	stem_loop	825	844	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01301	.	stop_codon	851	851	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01301	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_S01301	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S01301	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S01301	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S01301	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S01301	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S01301	.	terminator	730	809	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01301	.	stem_loop	741	784	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01301	.	engineered_region	730	809	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S01301
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagaggagaaatactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata