LOCUS       BBa_S01302              1066 bp    DNA     linear       07-Jun-2024
DEFINITION  Intermediate part from assembly 236
ACCESSION   BBa_S01302
VERSION     BBa_S01302.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     misc_feature    658..689
                     /label=LVA
     CDS             79..657
                     /label=luxI
     misc_feature    79..696
                     /label=BBa_C0061
     misc_feature    697..721
                     /label=Help:Barcodes
     CDS             79..696
                     /label=BBa_C0061
     stem_loop       741..784
                     /label=stem_loop
     misc_feature    730..809
                     /label=BBa_B0010
     terminator      730..809
                     /label=BBa_B0010
     stem_loop       825..844
                     /label=T7 TE
     misc_feature    851..851
                     /label=stop
     polyA_site      845..858
                     /label=polya
     misc_feature    818..858
                     /label=BBa_B0012
     terminator      818..858
                     /label=BBa_B0012
     CDS             867..954
                     /label=end of LacI coding region (inactive)
     misc_feature    867..1066
                     /label=BBa_R0010
     misc_feature    955..992
                     /label=CAP binding site
     promoter        1003..1008
                     /label=-35
     promoter        1027..1032
                     /label=-10
     misc_feature    1032..1066
                     /label=LacI binding site
     misc_feature    1039..1039
                     /label=start
     promoter        867..1066
                     /label=BBa_R0010
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca
      121 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag
      181 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa
      241 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca
      301 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc
      361 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata
      421 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt
      481 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag
      541 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact
      601 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct
      661 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct
      721 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      781 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      841 ggcctttctg cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc
      901 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      961 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1021 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacaca
//