LOCUS BBa_S01303 4026 bp DNA linear 22-Nov-2024 DEFINITION Intermediate part from assembly 236 ACCESSION BBa_S01303 VERSION BBa_S01303.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..72 /label=RBS-1\Strong RBS 65..69 /label=RBS misc_feature 58..72 /label=BBa_B0030 RBS 58..72 /label=BBa_B0030 misc_feature 658..689 /label=LVA CDS 79..657 /label=luxI misc_feature 79..696 /label=BBa_C0061 misc_feature 697..721 /label=Help:Barcodes CDS 79..696 /label=BBa_C0061 stem_loop 741..784 /label=stem_loop misc_feature 730..809 /label=BBa_B0010 terminator 730..809 /label=BBa_B0010 stem_loop 825..844 /label=T7 TE misc_feature 851..851 /label=stop polyA_site 845..858 /label=polya misc_feature 818..858 /label=BBa_B0012 terminator 818..858 /label=BBa_B0012 CDS 867..954 /label=end of LacI coding region (inactive) misc_feature 867..1066 /label=BBa_R0010 misc_feature 955..992 /label=CAP binding site promoter 1003..1008 /label=-35 promoter 1027..1032 /label=-10 misc_feature 1032..1066 /label=LacI binding site misc_feature 1039..1039 /label=start promoter 867..1066 /label=BBa_R0010 RBS 1075..1089 /label=RBS-1\Strong RBS 1082..1086 /label=RBS misc_feature 1075..1089 /label=BBa_B0030 RBS 1075..1089 /label=BBa_B0030 misc_feature 1096..1098 /label=start misc_feature 1879..1884 /label=2 misc_feature 1846..1878 /label=LVA CDS 1096..1845 /label=aiiA misc_feature 1096..1884 /label=BBa_C0060 misc_feature 1885..1909 /label=Help:Barcodes CDS 1096..1884 /label=BBa_C0060 stem_loop 1929..1972 /label=stem_loop misc_feature 1918..1997 /label=BBa_B0010 terminator 1918..1997 /label=BBa_B0010 stem_loop 2013..2032 /label=T7 TE misc_feature 2039..2039 /label=stop polyA_site 2033..2046 /label=polya misc_feature 2006..2046 /label=BBa_B0012 terminator 2006..2046 /label=BBa_B0012 misc_feature 2055..2073 /label=TetR 1 misc_feature 2055..2108 /label=BBa_R0040 promoter 2074..2079 /label=-35 misc_feature 2080..2098 /label=TetR 2 promoter 2097..2102 /label=-10 promoter 2055..2108 /label=BBa_R0040 RBS 2117..2131 /label=RBS-1\Strong RBS 2124..2128 /label=RBS misc_feature 2117..2131 /label=BBa_B0030 RBS 2117..2131 /label=BBa_B0030 CDS 2138..2899 /label=CFP (LVA) misc_feature 2856..2893 /label=SsrA misc_feature 2894..2899 /label=2 misc_feature 2206..2206 /label=A misc_feature 2138..2899 /label=BBa_E0022 misc_feature 2138..2899 /label=BBa_E0022 stem_loop 2919..2962 /label=stem_loop misc_feature 2908..2987 /label=BBa_B0010 terminator 2908..2987 /label=BBa_B0010 stem_loop 3003..3022 /label=T7 TE misc_feature 3029..3029 /label=stop polyA_site 3023..3036 /label=polya misc_feature 2996..3036 /label=BBa_B0012 terminator 2996..3036 /label=BBa_B0012 misc_feature 3045..3063 /label=TetR 1 misc_feature 3045..3098 /label=BBa_R0040 promoter 3064..3069 /label=-35 misc_feature 3070..3088 /label=TetR 2 promoter 3087..3092 /label=-10 promoter 3045..3098 /label=BBa_R0040 RBS 3107..3121 /label=RBS-1\Strong RBS 3114..3118 /label=RBS misc_feature 3107..3121 /label=BBa_B0030 RBS 3107..3121 /label=BBa_B0030 CDS 3128..3889 /label=YFP (LVA) misc_feature 3884..3889 /label=2 misc_feature 3845..3883 /label=SsrA misc_feature 3369..3369 /label=A (Q->L) misc_feature 3128..3889 /label=BBa_E0032 misc_feature 3128..3889 /label=BBa_E0032 stem_loop 3909..3952 /label=stem_loop misc_feature 3898..3977 /label=BBa_B0010 terminator 3898..3977 /label=BBa_B0010 stem_loop 3993..4012 /label=T7 TE misc_feature 4019..4019 /label=stop polyA_site 4013..4026 /label=polya misc_feature 3986..4026 /label=BBa_B0012 terminator 3986..4026 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt 61 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 121 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 181 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 241 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 301 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 361 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 421 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 481 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 541 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 601 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 661 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 721 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 781 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 841 ggcctttctg cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc 901 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 961 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 1021 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagattaaa 1081 gaggagaaat actagatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg 1141 ttggatcatt cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt 1201 tggtgttatc ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa 1261 agtgcagtta ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg 1321 aaaatgactg aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa 1381 gaccttcttt atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct 1441 tttataaata caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa 1501 gaatatttga aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat 1561 gaagtcgtac caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg 1621 ctattaattg agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg 1681 aaagagaatt ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct 1741 tcaattaaac gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat 1801 gatatagagc aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa 1861 aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca 1921 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 1981 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2041 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2101 ctgagcacta ctagagatta aagaggagaa atactagatg gtgagcaagg gcgaggagct 2161 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 2221 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 2281 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 2341 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 2401 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 2461 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 2521 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 2581 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 2641 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 2701 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 2761 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 2821 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta 2881 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2941 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 3001 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 3061 agattgacat ccctatcagt gatagagata ctgagcacta ctagagatta aagaggagaa 3121 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 3181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3301 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca 3361 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 3601 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3721 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3841 caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca 3901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 4021 tttata //