##gff-version 3
##sequence-region BBa_S01303 1 4026
BBa_S01303	.	CDS	1096	1884	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_S01303	.	start_codon	1096	1098	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01303	.	engineered_region	1096	1884	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01303	.	engineered_tag	1885	1909	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S01303	.	CDS	1096	1845	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01303	.	tag	1846	1878	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01303	.	stop_codon	1879	1884	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01303	.	ribosome_entry_site	2117	2131	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01303	.	ribosome_entry_site	2124	2128	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01303	.	engineered_region	2117	2131	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01303	.	ribosome_entry_site	2117	2131	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01303	.	terminator	2996	3036	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01303	.	engineered_region	2996	3036	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01303	.	stem_loop	3003	3022	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01303	.	stop_codon	3029	3029	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01303	.	polyA_site	3023	3036	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01303	.	promoter	2055	2108	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01303	.	promoter	2097	2102	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01303	.	promoter	2074	2079	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01303	.	engineered_region	2055	2108	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01303	.	non_covalent_binding_site	2080	2098	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01303	.	non_covalent_binding_site	2055	2073	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01303	.	promoter	867	1066	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01303	.	non_covalent_binding_site	955	992	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01303	.	engineered_region	867	1066	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01303	.	promoter	1027	1032	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01303	.	start_codon	1039	1039	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01303	.	non_covalent_binding_site	1032	1066	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01303	.	CDS	867	954	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01303	.	promoter	1003	1008	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01303	.	ribosome_entry_site	58	72	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01303	.	ribosome_entry_site	65	69	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01303	.	engineered_region	58	72	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01303	.	ribosome_entry_site	58	72	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01303	.	ribosome_entry_site	1075	1089	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01303	.	ribosome_entry_site	1082	1086	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01303	.	engineered_region	1075	1089	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01303	.	ribosome_entry_site	1075	1089	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01303	.	ribosome_entry_site	3107	3121	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01303	.	ribosome_entry_site	3114	3118	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01303	.	engineered_region	3107	3121	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01303	.	ribosome_entry_site	3107	3121	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01303	.	terminator	3898	3977	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01303	.	engineered_region	3898	3977	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01303	.	stem_loop	3909	3952	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01303	.	engineered_region	3128	3889	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01303	.	CDS	3128	3889	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01303	.	sequence_alteration	3369	3369	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01303	.	engineered_region	3128	3889	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01303	.	stop_codon	3884	3889	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01303	.	tag	3845	3883	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01303	.	terminator	730	809	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01303	.	engineered_region	730	809	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01303	.	stem_loop	741	784	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01303	.	terminator	3986	4026	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01303	.	engineered_region	3986	4026	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01303	.	stem_loop	3993	4012	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01303	.	stop_codon	4019	4019	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01303	.	polyA_site	4013	4026	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01303	.	CDS	79	696	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S01303	.	CDS	79	657	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S01303	.	engineered_tag	697	721	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S01303	.	tag	658	689	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S01303	.	engineered_region	79	696	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S01303	.	terminator	1918	1997	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01303	.	engineered_region	1918	1997	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01303	.	stem_loop	1929	1972	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01303	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_S01303	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S01303	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S01303	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S01303	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S01303	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S01303	.	promoter	3045	3098	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01303	.	promoter	3087	3092	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01303	.	promoter	3064	3069	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01303	.	engineered_region	3045	3098	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01303	.	non_covalent_binding_site	3070	3088	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01303	.	non_covalent_binding_site	3045	3063	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01303	.	engineered_region	2138	2899	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_S01303	.	CDS	2138	2899	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S01303	.	tag	2856	2893	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S01303	.	engineered_region	2138	2899	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S01303	.	point_mutation	2206	2206	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S01303	.	stop_codon	2894	2899	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S01303	.	terminator	2908	2987	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01303	.	engineered_region	2908	2987	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01303	.	stem_loop	2919	2962	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01303	.	terminator	2006	2046	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01303	.	engineered_region	2006	2046	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01303	.	stem_loop	2013	2032	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01303	.	stop_codon	2039	2039	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01303	.	polyA_site	2033	2046	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01303	.	terminator	818	858	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01303	.	engineered_region	818	858	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01303	.	stem_loop	825	844	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01303	.	stop_codon	851	851	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01303	.	polyA_site	845	858	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_S01303
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagaggagaaatactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaa
tacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcggg
cagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgt
atgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgacagtaaa
gaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaatta
ttagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtg
cagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaat
cgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcat
gcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaag
aatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttca
attattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattatta
acgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttat
cttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaag
gggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgc
tagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggc
cacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccg
gcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgacca
catgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgac
ggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgact
tcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaa
gcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactac
cagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagca
aagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacga
gctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggg
gtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatg
ccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccac
cttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgccc
gaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagg
gcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgga
gtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgc
cacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgc
tgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgct
ggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactac
gctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata