LOCUS BBa_S01304 4530 bp DNA linear 23-Dec-2024 DEFINITION Intermediate part from assembly 236 ACCESSION BBa_S01304 VERSION BBa_S01304.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..70 /label=RBS-3\Weak RBS 64..67 /label=RBS misc_feature 58..70 /label=BBa_B0032 RBS 58..70 /label=BBa_B0032 CDS 80..696 /label=tetR misc_feature 697..730 /label=SsrA misc_feature 737..761 /label=Help:Barcodes CDS 77..736 /label=BBa_C0040 stem_loop 781..824 /label=stem_loop misc_feature 770..849 /label=BBa_B0010 terminator 770..849 /label=BBa_B0010 stem_loop 865..884 /label=T7 TE misc_feature 891..891 /label=stop polyA_site 885..898 /label=polya misc_feature 858..898 /label=BBa_B0012 terminator 858..898 /label=BBa_B0012 misc_feature 907..925 /label=TetR 1 misc_feature 907..960 /label=BBa_R0040 promoter 926..931 /label=-35 misc_feature 932..950 /label=TetR 2 promoter 949..954 /label=-10 promoter 907..960 /label=BBa_R0040 RBS 969..981 /label=RBS-3\Weak RBS 975..978 /label=RBS misc_feature 969..981 /label=BBa_B0032 RBS 969..981 /label=BBa_B0032 misc_feature 2077..2115 /label=LVA CDS 988..2115 /label=lacI-LVA misc_feature 988..2115 /label=BBa_C0012 misc_feature 2116..2140 /label=Help:Barcodes CDS 988..2115 /label=BBa_C0012 stem_loop 2160..2203 /label=stem_loop misc_feature 2149..2228 /label=BBa_B0010 terminator 2149..2228 /label=BBa_B0010 stem_loop 2244..2263 /label=T7 TE misc_feature 2270..2270 /label=stop polyA_site 2264..2277 /label=polya misc_feature 2237..2277 /label=BBa_B0012 terminator 2237..2277 /label=BBa_B0012 CDS 2286..2373 /label=end of LacI coding region (inactive) misc_feature 2286..2485 /label=BBa_R0010 misc_feature 2374..2411 /label=CAP binding site promoter 2422..2427 /label=-35 promoter 2446..2451 /label=-10 misc_feature 2451..2485 /label=LacI binding site misc_feature 2458..2458 /label=start promoter 2286..2485 /label=BBa_R0010 RBS 2494..2506 /label=RBS-3\Weak RBS 2500..2503 /label=RBS misc_feature 2494..2506 /label=BBa_B0032 RBS 2494..2506 /label=BBa_B0032 CDS 2516..3223 /label=cI lambda misc_feature 3224..3256 /label=LVA misc_feature 3263..3287 /label=Help:Barcodes CDS 2513..3262 /label=BBa_C0051 stem_loop 3307..3350 /label=stem_loop misc_feature 3296..3375 /label=BBa_B0010 terminator 3296..3375 /label=BBa_B0010 stem_loop 3391..3410 /label=T7 TE misc_feature 3417..3417 /label=stop polyA_site 3411..3424 /label=polya misc_feature 3384..3424 /label=BBa_B0012 terminator 3384..3424 /label=BBa_B0012 misc_feature 3433..3452 /label=LuxR/HSL promoter 3547..3554 /label=-10 promoter 3521..3526 /label=-35 misc_feature 3560..3560 /label=start misc_feature 3562..3581 /label=Putative LuxR/HSL misc_feature 3433..3583 /label=BBa_R0063 promoter 3433..3583 /label=BBa_R0063 RBS 3592..3606 /label=RBS-1\Strong RBS 3599..3603 /label=RBS misc_feature 3592..3606 /label=BBa_B0030 RBS 3592..3606 /label=BBa_B0030 misc_feature 3613..3614 /label=prefix misc_feature 3786..3786 /label=T misc_feature 4104..4104 /label=A CDS 3613..4362 /label=luxR misc_feature 3613..4368 /label=BBa_C0062 misc_feature 4369..4393 /label=Help:Barcodes CDS 3613..4368 /label=BBa_C0062 stem_loop 4413..4456 /label=stem_loop misc_feature 4402..4481 /label=BBa_B0010 terminator 4402..4481 /label=BBa_B0010 stem_loop 4497..4516 /label=T7 TE misc_feature 4523..4523 /label=stop polyA_site 4517..4530 /label=polya misc_feature 4490..4530 /label=BBa_B0012 terminator 4490..4530 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggaaag tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga 121 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg 181 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt 241 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg 301 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga 361 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa 421 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag 481 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa 541 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga 601 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg 661 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc 721 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 781 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 841 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 901 ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac 961 tactagagtc acacaggaaa gtactagatg gtgaatgtga aaccagtaac gttatacgat 1021 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 1081 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 1141 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 1201 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 1261 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 1321 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 1381 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 1441 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 1501 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 1561 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 1621 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 1681 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 1741 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 1801 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 1861 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 1921 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 1981 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 2041 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct 2101 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa 2161 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2221 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 2281 tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 2341 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 2401 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 2461 tgtgagcgga taacaatttc acacatacta gagtcacaca ggaaagtact agatgagcac 2521 aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta 2581 tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat 2641 ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc 2701 cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag 2761 agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga 2821 gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac 2881 caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg 2941 gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga 3001 cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc 3061 cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt 3121 tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt 3181 ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa 3241 ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 3301 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 3361 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3421 tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg tattcccagc 3481 gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc attaccgttt 3541 taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga gattaaagag 3601 gagaaatact agatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt 3661 aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta 3721 cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt 3781 tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata 3841 aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata 3901 tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca 3961 ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt 4021 agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg 4081 aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat 4141 aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa 4201 ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc 4261 catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa 4321 gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca ctgatagtgc 4381 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 4441 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 4501 caccttcggg tgggcctttc tgcgtttata //