##gff-version 3
##sequence-region BBa_S01304 1 4530
BBa_S01304	.	terminator	2237	2277	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01304	.	stop_codon	2270	2270	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01304	.	engineered_region	2237	2277	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01304	.	stem_loop	2244	2263	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01304	.	polyA_site	2264	2277	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01304	.	CDS	988	2115	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_S01304	.	engineered_region	988	2115	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01304	.	engineered_tag	2116	2140	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01304	.	CDS	988	2115	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01304	.	tag	2077	2115	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01304	.	terminator	4402	4481	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01304	.	stem_loop	4413	4456	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01304	.	engineered_region	4402	4481	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01304	.	ribosome_entry_site	2494	2506	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S01304	.	engineered_region	2494	2506	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S01304	.	ribosome_entry_site	2494	2506	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S01304	.	ribosome_entry_site	2500	2503	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S01304	.	CDS	2513	3262	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S01304	.	tag	3224	3256	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01304	.	engineered_tag	3263	3287	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S01304	.	CDS	2516	3223	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01304	.	terminator	4490	4530	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01304	.	stop_codon	4523	4523	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01304	.	engineered_region	4490	4530	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01304	.	stem_loop	4497	4516	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01304	.	polyA_site	4517	4530	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01304	.	terminator	3384	3424	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01304	.	stop_codon	3417	3417	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01304	.	engineered_region	3384	3424	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01304	.	stem_loop	3391	3410	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01304	.	polyA_site	3411	3424	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01304	.	promoter	3433	3583	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_S01304	.	promoter	3521	3526	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S01304	.	start_codon	3560	3560	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S01304	.	non_covalent_binding_site	3562	3581	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S01304	.	non_covalent_binding_site	3433	3452	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S01304	.	engineered_region	3433	3583	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S01304	.	promoter	3547	3554	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S01304	.	promoter	907	960	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01304	.	promoter	926	931	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01304	.	non_covalent_binding_site	932	950	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01304	.	engineered_region	907	960	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01304	.	non_covalent_binding_site	907	925	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01304	.	promoter	949	954	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01304	.	ribosome_entry_site	58	70	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S01304	.	engineered_region	58	70	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S01304	.	ribosome_entry_site	58	70	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S01304	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S01304	.	ribosome_entry_site	3592	3606	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_S01304	.	ribosome_entry_site	3592	3606	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S01304	.	ribosome_entry_site	3599	3603	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S01304	.	engineered_region	3592	3606	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S01304	.	terminator	3296	3375	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01304	.	stem_loop	3307	3350	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01304	.	engineered_region	3296	3375	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01304	.	promoter	2286	2485	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01304	.	non_covalent_binding_site	2374	2411	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01304	.	promoter	2422	2427	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01304	.	engineered_region	2286	2485	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01304	.	CDS	2286	2373	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01304	.	promoter	2446	2451	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01304	.	start_codon	2458	2458	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01304	.	non_covalent_binding_site	2451	2485	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01304	.	ribosome_entry_site	969	981	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S01304	.	engineered_region	969	981	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S01304	.	ribosome_entry_site	969	981	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S01304	.	ribosome_entry_site	975	978	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S01304	.	CDS	77	736	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01304	.	engineered_tag	737	761	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01304	.	tag	697	730	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01304	.	CDS	80	696	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01304	.	terminator	770	849	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01304	.	stem_loop	781	824	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01304	.	engineered_region	770	849	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01304	.	CDS	3613	4368	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S01304	.	engineered_region	3613	4368	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S01304	.	CDS	3613	4362	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S01304	.	engineered_region	3613	3614	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S01304	.	point_mutation	3786	3786	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S01304	.	engineered_tag	4369	4393	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S01304	.	point_mutation	4104	4104	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S01304	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_S01304	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S01304	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S01304	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S01304	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S01304	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S01304	.	terminator	858	898	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01304	.	stop_codon	891	891	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01304	.	engineered_region	858	898	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01304	.	stem_loop	865	884	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01304	.	polyA_site	885	898	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01304	.	terminator	2149	2228	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01304	.	stem_loop	2160	2203	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01304	.	engineered_region	2149	2228	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S01304
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatg
tccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaaccc
gtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgc
cttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgt
aataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacag
aaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgc
actcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaa
acacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcct
tcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacga
aaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgataga
gatactgagcactactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcag
agtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaa
agtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctg
attggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaac
tgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgc
gcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaat
gttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgac
tgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcg
tctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagt
gccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatc
agatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacga
cgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagc
gtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa
aaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatac
gcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag
tgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatg
ttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgagcacaaaaaaga
aaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggctt
atcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcatta
aatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaa
tctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttca
ggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagcc
agtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacg
gaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtt
taccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgc
aatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaa
actacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattcc
cagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataaca
cgcaaaacttgcgacaaacaataggtaatactagagattaaagaggagaaatactagatgaaaaacataaatgccgacg
acacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaa
aatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagat
aattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaact
ccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagc
gaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgca
cattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctag
ttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagc
gtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccattta
accaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgatt
gcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata