##gff-version 3
##sequence-region BBa_S01373 1 1144
BBa_S01373	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01373	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01373	.	terminator	808	887	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01373	.	engineered_region	808	887	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01373	.	stem_loop	819	862	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01373	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S01373	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S01373	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S01373	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S01373	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S01373	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S01373	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S01373	.	promoter	945	1144	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01373	.	non_covalent_binding_site	1033	1070	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01373	.	non_covalent_binding_site	1110	1144	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01373	.	promoter	1081	1086	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01373	.	engineered_region	945	1144	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01373	.	promoter	1105	1110	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01373	.	start_codon	1117	1117	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01373	.	CDS	945	1032	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01373	.	terminator	896	936	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01373	.	stop_codon	929	929	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01373	.	polyA_site	923	936	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01373	.	stem_loop	903	922	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01373	.	engineered_region	896	936	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S01373
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaat
acgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc
agtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgta
tgttgtgtggaattgtgagcggataacaatttcacaca