##gff-version 3
##sequence-region BBa_S01374 1 1956
BBa_S01374	.	terminator	929	969	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01374	.	stop_codon	962	962	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01374	.	polyA_site	956	969	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01374	.	stem_loop	936	955	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01374	.	engineered_region	929	969	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01374	.	terminator	1828	1907	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01374	.	engineered_region	1828	1907	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01374	.	stem_loop	1839	1882	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01374	.	terminator	841	920	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01374	.	engineered_region	841	920	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01374	.	stem_loop	852	895	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01374	.	terminator	1916	1956	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01374	.	stop_codon	1949	1949	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01374	.	polyA_site	1943	1956	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01374	.	stem_loop	1923	1942	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01374	.	engineered_region	1916	1956	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01374	.	engineered_region	1058	1819	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01374	.	stop_codon	1814	1819	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01374	.	tag	1775	1813	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01374	.	engineered_region	1058	1819	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01374	.	sequence_alteration	1299	1299	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01374	.	CDS	1058	1819	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01374	.	promoter	978	1031	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01374	.	engineered_region	978	1031	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01374	.	promoter	997	1002	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01374	.	promoter	1020	1025	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01374	.	non_covalent_binding_site	978	996	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01374	.	non_covalent_binding_site	1003	1021	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01374	.	ribosome_entry_site	1040	1051	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01374	.	conserved_region	1044	1047	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01374	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01374	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01374	.	CDS	19	807	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_S01374	.	tag	769	801	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01374	.	stop_codon	802	807	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01374	.	CDS	19	768	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01374	.	engineered_region	19	807	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01374	.	start_codon	19	21	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01374	.	engineered_tag	808	832	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
>BBa_S01374
aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctg
ttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctat
tttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggtt
ttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttata
ttattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgc
tgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaa
ggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattg
agacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatt
tgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgtt
ttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacg
ctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactga
gcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctgg
tcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagct
gaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctg
caatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccagg
agcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaa
ccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagc
cacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacg
gcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccacta
cctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgcc
gccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata