BBa_S01374
type: ComponentDefinition
persistent identity: BBa S01374
display id: BBa_S01374
version: 1
was generated by: iGEM to SBOL conversion
description: Intermediate part from assembly 239
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
owned by: james
owned by: myers
discontinued: true
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0010
component: BBa_E0032
component: BBa_B0034
component: BBa_R0040
component: BBa_B0012
component: BBa_C0060
sequence annotation: BBa_B0034
sequence annotation: BBa_C0060
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_S01374_sequence/1

BBa_B0010
type: Component
persistent identity: component963191
display id: component963191
version: 1
top level: BBa_S01374
definition: BBa_B0010
access: public

BBa_E0032
type: Component
persistent identity: component963183
display id: component963183
version: 1
top level: BBa_S01374
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component963171
display id: component963171
version: 1
top level: BBa_S01374
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component963163
display id: component963163
version: 1
top level: BBa_S01374
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component963148
display id: component963148
version: 1
top level: BBa_S01374
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component963138
display id: component963138
version: 1
top level: BBa_S01374
definition: BBa_B0010
access: public

BBa_C0060
type: Component
persistent identity: component963130
display id: component963130
version: 1
top level: BBa_S01374
definition: aiiA
access: public

BBa_B0034
type: Component
persistent identity: component963115
display id: component963115
version: 1
top level: BBa_S01374
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component963201
display id: component963201
version: 1
top level: BBa_S01374
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation963115
display id: annotation963115
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963115/range963115/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01374/annotation963115/range963115/1
type: Range
persistent identity: range963115
display id: range963115
version: 1
top level: BBa_S01374
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation963130
display id: annotation963130
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963130/range963130/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_S01374/annotation963130/range963130/1
type: Range
persistent identity: range963130
display id: range963130
version: 1
top level: BBa_S01374
direction: direction/forward
start: 19
end: 807
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation963138
display id: annotation963138
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963138/range963138/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01374/annotation963138/range963138/1
type: Range
persistent identity: range963138
display id: range963138
version: 1
top level: BBa_S01374
direction: direction/forward
start: 841
end: 920
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation963148
display id: annotation963148
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963148/range963148/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01374/annotation963148/range963148/1
type: Range
persistent identity: range963148
display id: range963148
version: 1
top level: BBa_S01374
direction: direction/forward
start: 929
end: 969
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation963163
display id: annotation963163
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963163/range963163/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S01374/annotation963163/range963163/1
type: Range
persistent identity: range963163
display id: range963163
version: 1
top level: BBa_S01374
direction: direction/forward
start: 978
end: 1031
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation963171
display id: annotation963171
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963171/range963171/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01374/annotation963171/range963171/1
type: Range
persistent identity: range963171
display id: range963171
version: 1
top level: BBa_S01374
direction: direction/forward
start: 1040
end: 1051
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation963183
display id: annotation963183
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963183/range963183/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_S01374/annotation963183/range963183/1
type: Range
persistent identity: range963183
display id: range963183
version: 1
top level: BBa_S01374
direction: direction/forward
start: 1058
end: 1819
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation963191
display id: annotation963191
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963191/range963191/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01374/annotation963191/range963191/1
type: Range
persistent identity: range963191
display id: range963191
version: 1
top level: BBa_S01374
direction: direction/forward
start: 1828
end: 1907
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation963201
display id: annotation963201
version: 1
top level: BBa_S01374
location: https://synbiohub.org/public/igem/BBa_S01374/annotation963201/range963201/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01374/annotation963201/range963201/1
type: Range
persistent identity: range963201
display id: range963201
version: 1
top level: BBa_S01374
direction: direction/forward
start: 1916
end: 1956
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: start
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
top level: aiiA
direction: direction/forward
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
top level: aiiA
direction: direction/forward
start: 784
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
top level: aiiA
direction: direction/forward
start: 790
end: 814
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

https://synbiohub.org/public/igem/BBa_S01374_sequence/1
type: Sequence
persistent identity: BBa S01374 sequence
display id: BBa_S01374_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0060_sequence/1
type: Sequence
persistent identity: BBa C0060 sequence
display id: BBa_C0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z