LOCUS BBa_S01428 2511 bp DNA linear 25-Nov-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01428 VERSION BBa_S01428.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 CDS 1317..1404 /label=end of LacI coding region (inactive) misc_feature 1317..1516 /label=BBa_R0010 misc_feature 1405..1442 /label=CAP binding site promoter 1453..1458 /label=-35 promoter 1477..1482 /label=-10 misc_feature 1482..1516 /label=LacI binding site misc_feature 1489..1489 /label=start promoter 1317..1516 /label=BBa_R0010 misc_feature 1529..1532 /label=conserved RBS 1525..1536 /label=BBa_B0034 CDS 1546..2253 /label=cI lambda misc_feature 2254..2286 /label=LVA misc_feature 2293..2317 /label=Help:Barcodes CDS 1543..2292 /label=BBa_C0051 stem_loop 2337..2380 /label=stem_loop misc_feature 2326..2405 /label=BBa_B0010 terminator 2326..2405 /label=BBa_B0010 stem_loop 2421..2440 /label=T7 TE misc_feature 2447..2447 /label=stop polyA_site 2441..2454 /label=polya misc_feature 2414..2454 /label=BBa_B0012 terminator 2414..2454 /label=BBa_B0012 promoter 2500..2505 /label=-10 promoter 2477..2482 /label=-35 misc_feature 2487..2503 /label=OR1 misc_feature 2463..2479 /label=OR2 misc_feature 2463..2511 /label=BBa_R0051 promoter 2463..2511 /label=BBa_R0051 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat 1321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 1441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 1501 ataacaattt cacacatact agagaaagag gagaaatact agatgagcac aaaaaagaaa 1561 ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag 1621 aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca 1681 ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt 1741 gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac 1801 gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt 1861 ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac caaaggtgat 1921 gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt 1981 gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga cggaatgtta 2041 attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc cagacttggg 2101 ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt tttacaacca 2161 ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt ggggaaagtt 2221 atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa ctacgcttta 2281 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa 2341 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2401 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2461 agtaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg c // LOCUS BBa_S01377 2511 bp DNA linear 25-Nov-2024 DEFINITION Intermediate part from assembly 239 ACCESSION BBa_S01377 VERSION BBa_S01377.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 stem_loop 1191..1234 /label=stem_loop misc_feature 1180..1259 /label=BBa_B0010 terminator 1180..1259 /label=BBa_B0010 stem_loop 1275..1294 /label=T7 TE misc_feature 1301..1301 /label=stop polyA_site 1295..1308 /label=polya misc_feature 1268..1308 /label=BBa_B0012 terminator 1268..1308 /label=BBa_B0012 CDS 1317..1404 /label=end of LacI coding region (inactive) misc_feature 1317..1516 /label=BBa_R0010 misc_feature 1405..1442 /label=CAP binding site promoter 1453..1458 /label=-35 promoter 1477..1482 /label=-10 misc_feature 1482..1516 /label=LacI binding site misc_feature 1489..1489 /label=start promoter 1317..1516 /label=BBa_R0010 misc_feature 1529..1532 /label=conserved RBS 1525..1536 /label=BBa_B0034 CDS 1546..2253 /label=cI lambda misc_feature 2254..2286 /label=LVA misc_feature 2293..2317 /label=Help:Barcodes CDS 1543..2292 /label=BBa_C0051 stem_loop 2337..2380 /label=stem_loop misc_feature 2326..2405 /label=BBa_B0010 terminator 2326..2405 /label=BBa_B0010 stem_loop 2421..2440 /label=T7 TE misc_feature 2447..2447 /label=stop polyA_site 2441..2454 /label=polya misc_feature 2414..2454 /label=BBa_B0012 terminator 2414..2454 /label=BBa_B0012 promoter 2500..2505 /label=-10 promoter 2477..2482 /label=-35 misc_feature 2487..2503 /label=OR1 misc_feature 2463..2479 /label=OR2 misc_feature 2463..2511 /label=BBa_R0051 promoter 2463..2511 /label=BBa_R0051 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat 1321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 1441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 1501 ataacaattt cacacatact agagaaagag gagaaatact agatgagcac aaaaaagaaa 1561 ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag 1621 aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca 1681 ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt 1741 gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac 1801 gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt 1861 ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac caaaggtgat 1921 gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt 1981 gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga cggaatgtta 2041 attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc cagacttggg 2101 ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt tttacaacca 2161 ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt ggggaaagtt 2221 atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa ctacgcttta 2281 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa 2341 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2401 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2461 agtaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg c //