LOCUS       BBa_S01428              2511 bp    DNA     linear       25-Nov-2024
DEFINITION  Intermediate part from assembly 240
ACCESSION   BBa_S01428
VERSION     BBa_S01428.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     CDS             1317..1404
                     /label=end of LacI coding region (inactive)
     misc_feature    1317..1516
                     /label=BBa_R0010
     misc_feature    1405..1442
                     /label=CAP binding site
     promoter        1453..1458
                     /label=-35
     promoter        1477..1482
                     /label=-10
     misc_feature    1482..1516
                     /label=LacI binding site
     misc_feature    1489..1489
                     /label=start
     promoter        1317..1516
                     /label=BBa_R0010
     misc_feature    1529..1532
                     /label=conserved
     RBS             1525..1536
                     /label=BBa_B0034
     CDS             1546..2253
                     /label=cI lambda
     misc_feature    2254..2286
                     /label=LVA
     misc_feature    2293..2317
                     /label=Help:Barcodes
     CDS             1543..2292
                     /label=BBa_C0051
     stem_loop       2337..2380
                     /label=stem_loop
     misc_feature    2326..2405
                     /label=BBa_B0010
     terminator      2326..2405
                     /label=BBa_B0010
     stem_loop       2421..2440
                     /label=T7 TE
     misc_feature    2447..2447
                     /label=stop
     polyA_site      2441..2454
                     /label=polya
     misc_feature    2414..2454
                     /label=BBa_B0012
     terminator      2414..2454
                     /label=BBa_B0012
     promoter        2500..2505
                     /label=-10
     promoter        2477..2482
                     /label=-35
     misc_feature    2487..2503
                     /label=OR1
     misc_feature    2463..2479
                     /label=OR2
     misc_feature    2463..2511
                     /label=BBa_R0051
     promoter        2463..2511
                     /label=BBa_R0051
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat
     1321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1501 ataacaattt cacacatact agagaaagag gagaaatact agatgagcac aaaaaagaaa
     1561 ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag
     1621 aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca
     1681 ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt
     1741 gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac
     1801 gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt
     1861 ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac caaaggtgat
     1921 gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt
     1981 gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga cggaatgtta
     2041 attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc cagacttggg
     2101 ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt tttacaacca
     2161 ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt ggggaaagtt
     2221 atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa ctacgcttta
     2281 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
     2341 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2401 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     2461 agtaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg c
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