##gff-version 3
##sequence-region BBa_S01431 1 2559
BBa_S01431	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01431	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01431	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01431	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01431	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01431	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_S01431	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01431	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01431	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01431	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01431	.	terminator	2326	2405	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01431	.	engineered_region	2326	2405	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01431	.	stem_loop	2337	2380	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01431	.	promoter	1317	1516	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01431	.	non_covalent_binding_site	1405	1442	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01431	.	engineered_region	1317	1516	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01431	.	promoter	1477	1482	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01431	.	start_codon	1489	1489	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01431	.	non_covalent_binding_site	1482	1516	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01431	.	CDS	1317	1404	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01431	.	promoter	1453	1458	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01431	.	promoter	2463	2559	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S01431	.	promoter	2556	2559	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S01431	.	promoter	2533	2538	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S01431	.	engineered_region	2463	2559	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S01431	.	promoter	2520	2520	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S01431	.	non_covalent_binding_site	2519	2535	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S01431	.	non_covalent_binding_site	2543	2559	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S01431	.	promoter	2509	2514	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S01431	.	non_covalent_binding_site	2468	2487	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S01431	.	ribosome_entry_site	1525	1536	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01431	.	conserved_region	1529	1532	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01431	.	terminator	2414	2454	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01431	.	engineered_region	2414	2454	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01431	.	stem_loop	2421	2440	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01431	.	stop_codon	2447	2447	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01431	.	polyA_site	2441	2454	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01431	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01431	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01431	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01431	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01431	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01431	.	CDS	1543	2292	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S01431	.	tag	2254	2286	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01431	.	CDS	1546	2253	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01431	.	engineered_tag	2293	2317	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_S01431
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgc
gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgt
gagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgga
taacaatttcacacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagct
tgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagac
aagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgc
ttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggt
tagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgag
cttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttg
aggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccc
tgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagg
gatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtgg
ggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaata
acgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtg
cgtgttgactattttacctctggcggtgata