LOCUS       BBa_S01525               966 bp    DNA     linear       22-Nov-2024
DEFINITION  Intermediate part from assembly 240
ACCESSION   BBa_S01525
VERSION     BBa_S01525.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..750
                     /label=cI 434
     misc_feature    712..750
                     /label=LVA
     misc_feature    82..750
                     /label=BBa_C0052
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             82..750
                     /label=BBa_C0052
     stem_loop       795..838
                     /label=stem_loop
     misc_feature    784..863
                     /label=BBa_B0010
     terminator      784..863
                     /label=BBa_B0010
     stem_loop       879..898
                     /label=T7 TE
     misc_feature    905..905
                     /label=stop
     polyA_site      899..912
                     /label=polya
     misc_feature    872..912
                     /label=BBa_B0012
     terminator      872..912
                     /label=BBa_B0012
     terminator      784..912
                     /label=BBa_B0015
     misc_feature    921..963
                     /label=OR2
     misc_feature    928..941
                     /label=OR2
     misc_feature    950..963
                     /label=OR1
     promoter        921..926
                     /label=-35
     promoter        944..949
                     /label=-10
     promoter        922..927
                     /label=-35
     misc_feature    955..957
                     /label=start
     misc_feature    921..966
                     /label=BBa_R0052
     promoter        921..966
                     /label=BBa_R0052
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag
      121 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag
      181 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt
      241 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg
      301 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt
      361 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac
      421 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt
      481 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga
      541 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata
      601 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac
      661 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac
      721 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta tatactagag ttgacaaaca agatacattg tatgaaaata caagaaagtt
      961 tgttga
//