LOCUS       BBa_S01535              1007 bp    DNA     linear       23-Dec-2024
DEFINITION  Intermediate part from assembly 240
ACCESSION   BBa_S01535
VERSION     BBa_S01535.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             127..743
                     /label=tetR
     misc_feature    744..777
                     /label=SsrA
     misc_feature    784..808
                     /label=Help:Barcodes
     CDS             124..783
                     /label=BBa_C0040
     stem_loop       828..871
                     /label=stem_loop
     misc_feature    817..896
                     /label=BBa_B0010
     terminator      817..896
                     /label=BBa_B0010
     stem_loop       912..931
                     /label=T7 TE
     misc_feature    938..938
                     /label=stop
     polyA_site      932..945
                     /label=polya
     misc_feature    905..945
                     /label=BBa_B0012
     terminator      905..945
                     /label=BBa_B0012
     misc_feature    954..972
                     /label=TetR 1
     misc_feature    954..1007
                     /label=BBa_R0040
     promoter        973..978
                     /label=-35
     misc_feature    979..997
                     /label=TetR 2
     promoter        996..1001
                     /label=-10
     promoter        954..1007
                     /label=BBa_R0040
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
      181 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      241 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      301 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      361 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      421 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      481 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
      541 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      601 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
      661 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
      721 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa
      781 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
      841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat
      961 cagtgataga gattgacatc cctatcagtg atagagatac tgagcac
//