##gff-version 3
##sequence-region BBa_S0161 1 866
BBa_S0161	.	ribosome_entry_site	1	11	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_S0161	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_S0161	.	ribosome_entry_site	1	11	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_S0161	.	engineered_region	1	11	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_S0161	.	CDS	18	704	.	+	0	ID=BBa_C0053;Name=BBa_C0053
BBa_S0161	.	engineered_tag	705	729	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_S0161	.	tag	666	698	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_S0161	.	stop_codon	699	704	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_S0161	.	engineered_region	18	704	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_S0161	.	CDS	18	665	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_S0161	.	terminator	826	866	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S0161	.	stop_codon	859	859	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S0161	.	engineered_region	826	866	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S0161	.	stem_loop	833	852	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S0161	.	polyA_site	853	866	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S0161	.	terminator	738	817	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S0161	.	stem_loop	749	792	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S0161	.	engineered_region	738	817	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S0161
tcacacaggactactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagaca
agccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggg
gagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacg
ttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagc
tgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggctt
gatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaag
tcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatgga
tgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggc
gtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgat
agtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata