LOCUS BBa_S01663 3532 bp DNA linear 22-Nov-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01663 VERSION BBa_S01663.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..741 /label=BBa_C0040 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 misc_feature 912..930 /label=TetR 1 misc_feature 912..965 /label=BBa_R0040 promoter 931..936 /label=-35 misc_feature 937..955 /label=TetR 2 promoter 954..959 /label=-10 promoter 912..965 /label=BBa_R0040 misc_feature 978..981 /label=conserved RBS 974..985 /label=BBa_B0034 misc_feature 2081..2119 /label=LVA CDS 992..2119 /label=lacI-LVA misc_feature 992..2119 /label=BBa_C0012 misc_feature 2120..2144 /label=Help:Barcodes CDS 992..2119 /label=BBa_C0012 stem_loop 2164..2207 /label=stem_loop misc_feature 2153..2232 /label=BBa_B0010 terminator 2153..2232 /label=BBa_B0010 stem_loop 2248..2267 /label=T7 TE misc_feature 2274..2274 /label=stop polyA_site 2268..2281 /label=polya misc_feature 2241..2281 /label=BBa_B0012 terminator 2241..2281 /label=BBa_B0012 CDS 2290..2377 /label=end of LacI coding region (inactive) misc_feature 2290..2489 /label=BBa_R0010 misc_feature 2378..2415 /label=CAP binding site promoter 2426..2431 /label=-35 promoter 2450..2455 /label=-10 misc_feature 2455..2489 /label=LacI binding site misc_feature 2462..2462 /label=start promoter 2290..2489 /label=BBa_R0010 misc_feature 2502..2505 /label=conserved RBS 2498..2509 /label=BBa_B0034 CDS 2519..3226 /label=cI lambda misc_feature 3227..3259 /label=LVA misc_feature 3266..3290 /label=Help:Barcodes CDS 2516..3265 /label=BBa_C0051 stem_loop 3310..3353 /label=stem_loop misc_feature 3299..3378 /label=BBa_B0010 terminator 3299..3378 /label=BBa_B0010 stem_loop 3394..3413 /label=T7 TE misc_feature 3420..3420 /label=stop polyA_site 3414..3427 /label=polya misc_feature 3387..3427 /label=BBa_B0012 terminator 3387..3427 /label=BBa_B0012 misc_feature 3436..3532 /label=BBa_R0065 misc_feature 3441..3460 /label=Lux Box promoter 3482..3487 /label=-10 misc_feature 3492..3508 /label=OR2 cI promoter 3493..3493 /label=lux p(R) start promoter 3506..3511 /label=-35 misc_feature 3516..3532 /label=OR1 cI promoter 3529..3532 /label=-10 promoter 3436..3532 /label=BBa_R0065 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 961 agcactacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata 1021 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 1081 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 1141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 1201 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 1261 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 1321 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 1381 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 1441 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 1501 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 1561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 1621 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 1681 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 1741 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 1801 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 1861 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 1921 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 1981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 2041 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2281 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 2341 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 2401 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 2461 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatgag 2521 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat 2581 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg 2641 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa 2701 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc 2761 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta 2821 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt 2881 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt 2941 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc 3001 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat 3061 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt 3121 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt 3181 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga 3241 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc 3301 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3361 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3421 gtttatatac tagagtaagc acctgtagga tcgtacaggt ttacgcaaga aaatggtttg 3481 ttatagtcga ataacaccgt gcgtgttgac tattttacct ctggcggtga ta //