LOCUS       BBa_S01664              2428 bp    DNA     linear       22-Nov-2024
DEFINITION  --Specify Parts List--
ACCESSION   BBa_S01664
VERSION     BBa_S01664.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..791
                     /label=cI lambda
     misc_feature    792..824
                     /label=LVA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..830
                     /label=BBa_C0051
     stem_loop       875..918
                     /label=stem_loop
     misc_feature    864..943
                     /label=BBa_B0010
     terminator      864..943
                     /label=BBa_B0010
     stem_loop       959..978
                     /label=T7 TE
     misc_feature    985..985
                     /label=stop
     polyA_site      979..992
                     /label=polya
     misc_feature    952..992
                     /label=BBa_B0012
     terminator      952..992
                     /label=BBa_B0012
     promoter        1038..1043
                     /label=-10
     promoter        1015..1020
                     /label=-35
     misc_feature    1025..1041
                     /label=OR1
     misc_feature    1001..1017
                     /label=OR2
     misc_feature    1001..1049
                     /label=BBa_R0051
     promoter        1001..1049
                     /label=BBa_R0051
     misc_feature    63..1049
                     /label=BBa_Q04510
     misc_feature    1062..1065
                     /label=conserved
     RBS             1058..1069
                     /label=BBa_B0034
     misc_feature    2165..2203
                     /label=LVA
     CDS             1076..2203
                     /label=lacI-LVA
     misc_feature    1076..2203
                     /label=BBa_C0012
     misc_feature    2204..2228
                     /label=Help:Barcodes
     CDS             1076..2203
                     /label=BBa_C0012
     stem_loop       2248..2291
                     /label=stem_loop
     misc_feature    2237..2316
                     /label=BBa_B0010
     terminator      2237..2316
                     /label=BBa_B0010
     stem_loop       2332..2351
                     /label=T7 TE
     misc_feature    2358..2358
                     /label=stop
     polyA_site      2352..2365
                     /label=polya
     misc_feature    2325..2365
                     /label=BBa_B0012
     terminator      2325..2365
                     /label=BBa_B0012
     terminator      2237..2365
                     /label=BBa_B0015
     misc_feature    2376..2392
                     /label=lac O1
     promoter        2393..2398
                     /label=-35
     misc_feature    2399..2415
                     /label=lac O1
     promoter        2416..2421
                     /label=-10
     misc_feature    2374..2427
                     /label=BBa_R0011
     promoter        2374..2427
                     /label=BBa_R0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg
      121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc
      181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg
      241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg
      301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta
      361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa
      481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac
      541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg
      601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga
      661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga
      721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag
      781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
      841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact
     1021 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatggt
     1081 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
     1141 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
     1201 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
     1261 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
     1321 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
     1381 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
     1441 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
     1501 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
     1561 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
     1621 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
     1681 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
     1741 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
     1801 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
     1861 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
     1921 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
     1981 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     2041 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
     2101 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2161 gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg
     2221 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2281 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2341 tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac aattgacatt
     2401 gtgagcggat aacaagatac tgagcaca
//