LOCUS BBa_S01674 3387 bp DNA linear 22-Nov-2024 DEFINITION R0062.Q04400.Q04121.Q04511 ACCESSION BBa_S01674 VERSION BBa_S01674.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..741 /label=BBa_C0040 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 misc_feature 912..930 /label=TetR 1 misc_feature 912..965 /label=BBa_R0040 promoter 931..936 /label=-35 misc_feature 937..955 /label=TetR 2 promoter 954..959 /label=-10 promoter 912..965 /label=BBa_R0040 misc_feature 978..981 /label=conserved RBS 974..985 /label=BBa_B0034 misc_feature 2081..2119 /label=LVA CDS 992..2119 /label=lacI-LVA misc_feature 992..2119 /label=BBa_C0012 misc_feature 2120..2144 /label=Help:Barcodes CDS 992..2119 /label=BBa_C0012 stem_loop 2164..2207 /label=stem_loop misc_feature 2153..2232 /label=BBa_B0010 terminator 2153..2232 /label=BBa_B0010 stem_loop 2248..2267 /label=T7 TE misc_feature 2274..2274 /label=stop polyA_site 2268..2281 /label=polya misc_feature 2241..2281 /label=BBa_B0012 terminator 2241..2281 /label=BBa_B0012 misc_feature 2292..2308 /label=lac O1 promoter 2309..2314 /label=-35 misc_feature 2315..2331 /label=lac O1 promoter 2332..2337 /label=-10 misc_feature 2290..2343 /label=BBa_R0011 promoter 2290..2343 /label=BBa_R0011 misc_feature 2357..2360 /label=conserved RBS 2353..2364 /label=BBa_B0034 CDS 2374..3081 /label=cI lambda misc_feature 3082..3114 /label=LVA misc_feature 3121..3145 /label=Help:Barcodes CDS 2371..3120 /label=BBa_C0051 stem_loop 3165..3208 /label=stem_loop misc_feature 3154..3233 /label=BBa_B0010 terminator 3154..3233 /label=BBa_B0010 stem_loop 3249..3268 /label=T7 TE misc_feature 3275..3275 /label=stop polyA_site 3269..3282 /label=polya misc_feature 3242..3282 /label=BBa_B0012 terminator 3242..3282 /label=BBa_B0012 misc_feature 3291..3387 /label=BBa_R0065 misc_feature 3296..3315 /label=Lux Box promoter 3337..3342 /label=-10 misc_feature 3347..3363 /label=OR2 cI promoter 3348..3348 /label=lux p(R) start promoter 3361..3366 /label=-35 misc_feature 3371..3387 /label=OR1 cI promoter 3384..3387 /label=-10 promoter 3291..3387 /label=BBa_R0065 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 961 agcactacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata 1021 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 1081 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 1141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 1201 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 1261 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 1321 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 1381 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 1441 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 1501 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 1561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 1621 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 1681 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 1741 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 1801 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 1861 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 1921 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 1981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 2041 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2281 atactagaga attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag 2341 cacatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa 2401 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt 2461 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct 2521 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc 2581 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa 2641 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt 2701 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg 2761 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc 2821 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac 2881 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt 2941 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag 3001 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag 3061 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa 3121 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 3181 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3241 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag taagcacctg 3301 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac accgtgcgtg 3361 ttgactattt tacctctggc ggtgata //