##gff-version 3
##sequence-region BBa_S01675 1 1827
BBa_S01675	.	ribosome_entry_site	911	922	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01675	.	conserved_region	915	918	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01675	.	terminator	1699	1778	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01675	.	stem_loop	1710	1753	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01675	.	engineered_region	1699	1778	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01675	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01675	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01675	.	promoter	849	902	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01675	.	non_covalent_binding_site	849	867	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01675	.	engineered_region	849	902	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01675	.	promoter	868	873	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01675	.	promoter	891	896	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01675	.	non_covalent_binding_site	874	892	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01675	.	engineered_region	929	1690	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_S01675	.	CDS	929	1690	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01675	.	tag	1646	1684	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01675	.	sequence_alteration	1170	1170	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01675	.	engineered_region	929	1690	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01675	.	stop_codon	1685	1690	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01675	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01675	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01675	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01675	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01675	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01675	.	terminator	1787	1827	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01675	.	stem_loop	1794	1813	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01675	.	engineered_region	1787	1827	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01675	.	stop_codon	1820	1820	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01675	.	polyA_site	1814	1827	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01675	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01675	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01675	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01675	.	CDS	19	678	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01675	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01675	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01675	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_S01675
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgagga
gctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgag
ggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccca
ccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaa
gtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgag
gtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgg
ggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaa
cttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgac
ggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatc
acatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaa
cgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata