LOCUS BBa_S01816 3282 bp DNA linear 06-Nov-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01816 VERSION BBa_S01816.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..750 /label=cI 434 misc_feature 712..750 /label=LVA misc_feature 82..750 /label=BBa_C0052 misc_feature 751..775 /label=Help:Barcodes CDS 82..750 /label=BBa_C0052 stem_loop 795..838 /label=stem_loop misc_feature 784..863 /label=BBa_B0010 terminator 784..863 /label=BBa_B0010 stem_loop 879..898 /label=T7 TE misc_feature 905..905 /label=stop polyA_site 899..912 /label=polya misc_feature 872..912 /label=BBa_B0012 terminator 872..912 /label=BBa_B0012 terminator 784..912 /label=BBa_B0015 misc_feature 921..963 /label=OR2 misc_feature 928..941 /label=OR2 misc_feature 950..963 /label=OR1 promoter 921..926 /label=-35 promoter 944..949 /label=-10 promoter 922..927 /label=-35 misc_feature 955..957 /label=start misc_feature 921..966 /label=BBa_R0052 promoter 921..966 /label=BBa_R0052 misc_feature 979..982 /label=conserved RBS 975..986 /label=BBa_B0034 CDS 993..1640 /label=cII p22 misc_feature 1641..1673 /label=LVA misc_feature 1674..1679 /label=stop misc_feature 993..1679 /label=BBa_C0053 misc_feature 1680..1704 /label=Help:Barcodes CDS 993..1679 /label=BBa_C0053 stem_loop 1724..1767 /label=stem_loop misc_feature 1713..1792 /label=BBa_B0010 terminator 1713..1792 /label=BBa_B0010 stem_loop 1808..1827 /label=T7 TE misc_feature 1834..1834 /label=stop polyA_site 1828..1841 /label=polya misc_feature 1801..1841 /label=BBa_B0012 terminator 1801..1841 /label=BBa_B0012 misc_feature 1850..1852 /label=OR3 misc_feature 1860..1877 /label=OR2 misc_feature 1883..1900 /label=OR1 promoter 1867..1872 /label=-35 promoter 1857..1862 /label=-35 promoter 1879..1884 /label=-10 misc_feature 1850..1903 /label=BBa_R0053 promoter 1850..1903 /label=BBa_R0053 misc_feature 975..1903 /label=BBa_Q04530 misc_feature 1916..1919 /label=conserved RBS 1912..1923 /label=BBa_B0034 misc_feature 3019..3057 /label=LVA CDS 1930..3057 /label=lacI-LVA misc_feature 1930..3057 /label=BBa_C0012 misc_feature 3058..3082 /label=Help:Barcodes CDS 1930..3057 /label=BBa_C0012 stem_loop 3102..3145 /label=stem_loop misc_feature 3091..3170 /label=BBa_B0010 terminator 3091..3170 /label=BBa_B0010 stem_loop 3186..3205 /label=T7 TE misc_feature 3212..3212 /label=stop polyA_site 3206..3219 /label=polya misc_feature 3179..3219 /label=BBa_B0012 terminator 3179..3219 /label=BBa_B0012 terminator 3091..3219 /label=BBa_B0015 misc_feature 3230..3246 /label=lac O1 promoter 3247..3252 /label=-35 misc_feature 3253..3269 /label=lac O1 promoter 3270..3275 /label=-10 misc_feature 3228..3281 /label=BBa_R0011 promoter 3228..3281 /label=BBa_R0011 misc_feature 1912..3282 /label=BBa_Q04121 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag 121 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag 181 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt 241 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg 301 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt 361 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac 421 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt 481 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga 541 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata 601 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac 661 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac 721 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 901 tctgcgttta tatactagag ttgacaaaca agatacattg tatgaaaata caagaaagtt 961 tgttgatact agagaaagag gagaaatact agatgaatac acaattgatg ggtgagcgta 1021 ttcgcgctcg aagaaaaaaa ctcaagatta gacaagccgc tcttggtaag atggtgggag 1081 tgtctaatgt tgcaatatcg caatgggagc gctcggagac tgagccaaat ggggagaacc 1141 tgttggcact ttcgaaggct cttcagtgct cccctgacta tttgctgaaa ggagatttaa 1201 gccagacaaa cgttgcctat catagtaggc atgagccaag aggatcatac cctcttatca 1261 gttgggtaag cgcagggcaa tggatggaag ctgtagaacc ttatcacaag cgcgcgatag 1321 agaactggca cgacaccact gtagattgtt cagaagattc attttggctt gatgtccaag 1381 gtgactctat gacagcaccg gcagggttaa gcattccaga aggaatgata attctggttg 1441 atcccgaagt cgaaccaaga aacggcaagc tggttgttgc aaaattagaa ggtgaaaacg 1501 aggccacatt caaaaaatta gttatggatg caggccgaaa gtttttaaaa ccattaaacc 1561 cacaatatcc gatgatagaa atcaacggaa actgcaaaat cattggcgta gttgttgacg 1621 caaaactcgc aaatcttcca gctgcaaacg acgaaaacta cgctttagta gcttaataac 1681 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 1741 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1801 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga ataaacttga 1861 ctaaagattc ctttagtaga taatttaagt gttctttaat ttctactaga gaaagaggag 1921 aaatactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 1981 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 2041 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 2101 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 2161 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 2221 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 2281 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 2341 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 2401 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 2461 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 2521 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 2581 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 2641 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 2701 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 2761 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 2821 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 2881 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 2941 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 3001 cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 3061 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 3121 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3181 acactggctc accttcgggt gggcctttct gcgtttatat actagagaat tgtgagcgga 3241 taacaattga cattgtgagc ggataacaag atactgagca ca //