BBa_S01816
type: ComponentDefinition
persistent identity: BBa S01816
display id: BBa_S01816
version: 1
was generated by: iGEM to SBOL conversion
description: Intermediate part from assembly 240
created: 2004-01-15T12:00:00Z
modified: 2015-05-08T01:14:19Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Randy Rettberg
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_R0011
component: BBa_B0015
component: BBa_C0052
component: BBa_Q04530
component: BBa_R0052
component: BBa_Q04121
component: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0052
sequence annotation: BBa_B0015
sequence annotation: BBa_R0052
sequence annotation: BBa_Q04530
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_S01816_sequence/1

BBa_R0011
type: Component
persistent identity: component2235400
display id: component2235400
version: 1
top level: BBa_S01816
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2235417
display id: component2235417
version: 1
top level: BBa_S01816
definition: BBa_B0015
access: public

BBa_C0052
type: Component
persistent identity: component2235408
display id: component2235408
version: 1
top level: BBa_S01816
definition: cI 434
access: public

BBa_Q04530
type: Component
persistent identity: component2235446
display id: component2235446
version: 1
top level: BBa_S01816
definition: BBa_Q04530
access: public

BBa_R0052
type: Component
persistent identity: component2235420
display id: component2235420
version: 1
top level: BBa_S01816
definition: cI 434
access: public

BBa_Q04121
type: Component
persistent identity: component2235465
display id: component2235465
version: 1
top level: BBa_S01816
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component2235406
display id: component2235406
version: 1
top level: BBa_S01816
definition: BBa_B0034
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2235400
display id: annotation2235400
version: 1
top level: BBa_S01816
location: https://synbiohub.org/public/igem/BBa_S01816/annotation2235400/range2235400/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_S01816/annotation2235400/range2235400/1
type: Range
persistent identity: range2235400
display id: range2235400
version: 1
top level: BBa_S01816
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2235406
display id: annotation2235406
version: 1
top level: BBa_S01816
location: https://synbiohub.org/public/igem/BBa_S01816/annotation2235406/range2235406/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01816/annotation2235406/range2235406/1
type: Range
persistent identity: range2235406
display id: range2235406
version: 1
top level: BBa_S01816
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation2235408
display id: annotation2235408
version: 1
top level: BBa_S01816
location: https://synbiohub.org/public/igem/BBa_S01816/annotation2235408/range2235408/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_S01816/annotation2235408/range2235408/1
type: Range
persistent identity: range2235408
display id: range2235408
version: 1
top level: BBa_S01816
direction: direction/forward
start: 82
end: 750
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2235417
display id: annotation2235417
version: 1
top level: BBa_S01816
location: https://synbiohub.org/public/igem/BBa_S01816/annotation2235417/range2235417/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S01816/annotation2235417/range2235417/1
type: Range
persistent identity: range2235417
display id: range2235417
version: 1
top level: BBa_S01816
direction: direction/reverse
start: 784
end: 912
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation2235420
display id: annotation2235420
version: 1
top level: BBa_S01816
location: https://synbiohub.org/public/igem/BBa_S01816/annotation2235420/range2235420/1
component: BBa_R0052

https://synbiohub.org/public/igem/BBa_S01816/annotation2235420/range2235420/1
type: Range
persistent identity: range2235420
display id: range2235420
version: 1
top level: BBa_S01816
direction: direction/forward
start: 921
end: 966
orientation: inline

BBa_Q04530
type: SequenceAnnotation
persistent identity: annotation2235446
display id: annotation2235446
version: 1
top level: BBa_S01816
location: https://synbiohub.org/public/igem/BBa_S01816/annotation2235446/range2235446/1
component: BBa_Q04530

https://synbiohub.org/public/igem/BBa_S01816/annotation2235446/range2235446/1
type: Range
persistent identity: range2235446
display id: range2235446
version: 1
top level: BBa_S01816
direction: direction/reverse
start: 975
end: 1903
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2235465
display id: annotation2235465
version: 1
top level: BBa_S01816
location: https://synbiohub.org/public/igem/BBa_S01816/annotation2235465/range2235465/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_S01816/annotation2235465/range2235465/1
type: Range
persistent identity: range2235465
display id: range2235465
version: 1
top level: BBa_S01816
direction: direction/reverse
start: 1912
end: 3282
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0052
sequence annotation: cI 434
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
top level: cI 434
direction: direction/forward
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
top level: cI 434
direction: direction/forward
start: 670
end: 694
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa Q04121
display id: BBa_Q04121
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0012.B0015.R0011)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LacI QPI with strong RBS, hybrid promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0015
component: BBa_C0012
component: BBa_R0011
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_Q04121_sequence/1

BBa_B0015
type: Component
persistent identity: component2221145
display id: component2221145
version: 1
top level: lacI
definition: BBa_B0015
access: public

BBa_C0012
type: Component
persistent identity: component2221136
display id: component2221136
version: 1
top level: lacI
definition: lacI
access: public

BBa_R0011
type: Component
persistent identity: component2221146
display id: component2221146
version: 1
top level: lacI
definition: lacI+pL
access: public

BBa_B0034
type: Component
persistent identity: component2221134
display id: component2221134
version: 1
top level: lacI
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221134
display id: annotation2221134
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221134/range2221134/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221134/range2221134/1
type: Range
persistent identity: range2221134
display id: range2221134
version: 1
top level: lacI
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2221136
display id: annotation2221136
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221136/range2221136/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221136/range2221136/1
type: Range
persistent identity: range2221136
display id: range2221136
version: 1
top level: lacI
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221145
display id: annotation2221145
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221145/range2221145/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221145/range2221145/1
type: Range
persistent identity: range2221145
display id: range2221145
version: 1
top level: lacI
direction: direction/reverse
start: 1180
end: 1308
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221146
display id: annotation2221146
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_Q04121/annotation2221146/range2221146/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_Q04121/annotation2221146/range2221146/1
type: Range
persistent identity: range2221146
display id: range2221146
version: 1
top level: lacI
direction: direction/forward
start: 1317
end: 1370
orientation: inline

BBa_Q04530
type: ComponentDefinition
persistent identity: BBa Q04530
display id: BBa_Q04530
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0053.B0015.R0053)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: p22 c2 QPI w/ strong RBS
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Inverter
role: SO:0000804
component: BBa_B0010
component: BBa_C0053
component: BBa_B0034
component: BBa_R0053
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_Q04530_sequence/1

BBa_B0010
type: Component
persistent identity: component953065
display id: component953065
version: 1
top level: BBa_Q04530
definition: BBa_B0010
access: public

BBa_C0053
type: Component
persistent identity: component953057
display id: component953057
version: 1
top level: BBa_Q04530
definition: c2 P22
access: public

BBa_B0034
type: Component
persistent identity: component953045
display id: component953045
version: 1
top level: BBa_Q04530
definition: BBa_B0034
access: public

BBa_R0053
type: Component
persistent identity: component953094
display id: component953094
version: 1
top level: BBa_Q04530
definition: cII p22
access: public

BBa_B0012
type: Component
persistent identity: component953075
display id: component953075
version: 1
top level: BBa_Q04530
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation953045
display id: annotation953045
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953045/range953045/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04530/annotation953045/range953045/1
type: Range
persistent identity: range953045
display id: range953045
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation953057
display id: annotation953057
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953057/range953057/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_Q04530/annotation953057/range953057/1
type: Range
persistent identity: range953057
display id: range953057
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 19
end: 705
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation953065
display id: annotation953065
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953065/range953065/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04530/annotation953065/range953065/1
type: Range
persistent identity: range953065
display id: range953065
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 739
end: 818
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation953075
display id: annotation953075
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953075/range953075/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04530/annotation953075/range953075/1
type: Range
persistent identity: range953075
display id: range953075
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 827
end: 867
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation953094
display id: annotation953094
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953094/range953094/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_Q04530/annotation953094/range953094/1
type: Range
persistent identity: range953094
display id: range953094
version: 1
top level: BBa_Q04530
direction: direction/forward
start: 876
end: 929
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (434 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: OR2
sequence annotation: -35
sequence annotation: BBa_R0052
sequence annotation: -10
sequence annotation: OR1
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
top level: cI 434
direction: direction/forward
start: 2
end: 7
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
top level: cI 434
direction: direction/forward
start: 8
end: 21
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
top level: cI 434
direction: direction/forward
start: 24
end: 29
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
top level: cI 434
direction: direction/forward
start: 30
end: 43
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
top level: cI 434
direction: direction/forward
start: 35
end: 37
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: iGEM to SBOL conversion
description: c2 repressor from Salmonella phage P22 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cII p22
sequence annotation: BBa_C0053
sequence annotation: LVA
sequence annotation: stop
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 648
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
top level: c2 P22
direction: direction/forward
start: 649
end: 681
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
top level: c2 P22
direction: direction/forward
start: 682
end: 687
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
top level: c2 P22
direction: direction/forward
start: 688
end: 712
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0053
sequence annotation: OR3
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

https://synbiohub.org/public/igem/BBa_S01816_sequence/1
type: Sequence
persistent identity: BBa S01816 sequence
display id: BBa_S01816_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactagagaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0052_sequence/1
type: Sequence
persistent identity: BBa C0052 sequence
display id: BBa_C0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04121_sequence/1
type: Sequence
persistent identity: BBa Q04121 sequence
display id: BBa_Q04121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04530_sequence/1
type: Sequence
persistent identity: BBa Q04530 sequence
display id: BBa_Q04530_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0052_sequence/1
type: Sequence
persistent identity: BBa R0052 sequence
display id: BBa_R0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0053_sequence/1
type: Sequence
persistent identity: BBa C0053 sequence
display id: BBa_C0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z