LOCUS BBa_S01819 1094 bp DNA linear 22-Nov-2024 DEFINITION --Specify Parts List-- ACCESSION BBa_S01819 VERSION BBa_S01819.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..84 /label=start misc_feature 865..870 /label=2 misc_feature 832..864 /label=LVA CDS 82..831 /label=aiiA misc_feature 82..870 /label=BBa_C0060 misc_feature 871..895 /label=Help:Barcodes CDS 82..870 /label=BBa_C0060 stem_loop 915..958 /label=stem_loop misc_feature 904..983 /label=BBa_B0010 terminator 904..983 /label=BBa_B0010 stem_loop 999..1018 /label=T7 TE misc_feature 1025..1025 /label=stop polyA_site 1019..1032 /label=polya misc_feature 992..1032 /label=BBa_B0012 terminator 992..1032 /label=BBa_B0012 misc_feature 64..1032 /label=BBa_I0460 misc_feature 1041..1059 /label=TetR 1 misc_feature 1041..1094 /label=BBa_R0040 promoter 1060..1065 /label=-35 misc_feature 1066..1084 /label=TetR 2 promoter 1083..1088 /label=-10 promoter 1041..1094 /label=BBa_R0040 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt 121 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta 181 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg 241 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt 301 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag 361 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat 421 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat 481 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt 541 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat 601 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg 661 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct 721 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt 781 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac 841 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta 901 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 961 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 1021 tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct atcagtgata 1081 gagatactga gcac //