##gff-version 3
##sequence-region BBa_S01839 1 3282
BBa_S01839	.	promoter	921	966	.	+	0	ID=BBa_R0052;Name=BBa_R0052
BBa_S01839	.	promoter	921	926	.	+	0	ID=annotation2029;Name=-35;Parent=BBa_R0052
BBa_S01839	.	promoter	944	949	.	+	0	ID=annotation2030;Name=-10;Parent=BBa_R0052
BBa_S01839	.	non_covalent_binding_site	921	963	.	+	0	ID=annotation2026;Name=OR2;Parent=BBa_R0052
BBa_S01839	.	promoter	922	927	.	+	0	ID=annotation2031;Name=-35;Parent=BBa_R0052
BBa_S01839	.	non_covalent_binding_site	928	941	.	+	0	ID=annotation2027;Name=OR2;Parent=BBa_R0052
BBa_S01839	.	start_codon	955	957	.	+	0	ID=annotation2032;Name=start;Parent=BBa_R0052
BBa_S01839	.	engineered_region	921	966	.	+	0	ID=annotation7068;Name=BBa_R0052;Parent=BBa_R0052
BBa_S01839	.	non_covalent_binding_site	950	963	.	+	0	ID=annotation2028;Name=OR1;Parent=BBa_R0052
BBa_S01839	.	CDS	82	750	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_S01839	.	engineered_region	82	750	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_S01839	.	engineered_tag	751	775	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_S01839	.	tag	712	750	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_S01839	.	CDS	82	750	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_S01839	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S01839	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01839	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01839	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01839	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01839	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01839	.	engineered_region	975	1903	.	+	0	ID=BBa_Q04530;Name=BBa_Q04530
BBa_S01839	.	promoter	1850	1903	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_Q04530
BBa_S01839	.	non_covalent_binding_site	1850	1852	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_S01839	.	promoter	1879	1884	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_S01839	.	non_covalent_binding_site	1860	1877	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_S01839	.	promoter	1857	1862	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_S01839	.	non_covalent_binding_site	1883	1900	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_S01839	.	engineered_region	1850	1903	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_S01839	.	promoter	1867	1872	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_S01839	.	terminator	1801	1841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04530
BBa_S01839	.	stem_loop	1808	1827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01839	.	polyA_site	1828	1841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01839	.	stop_codon	1834	1834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01839	.	engineered_region	1801	1841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01839	.	CDS	993	1679	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_Q04530
BBa_S01839	.	tag	1641	1673	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_S01839	.	engineered_region	993	1679	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_S01839	.	engineered_tag	1680	1704	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_S01839	.	stop_codon	1674	1679	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_S01839	.	CDS	993	1640	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_S01839	.	terminator	1713	1792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04530
BBa_S01839	.	engineered_region	1713	1792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01839	.	stem_loop	1724	1767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01839	.	ribosome_entry_site	975	986	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04530
BBa_S01839	.	conserved_region	979	982	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01839	.	terminator	784	912	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S01839	.	terminator	872	912	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01839	.	stem_loop	879	898	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01839	.	polyA_site	899	912	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01839	.	stop_codon	905	905	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01839	.	engineered_region	872	912	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01839	.	terminator	784	863	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01839	.	engineered_region	784	863	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01839	.	stem_loop	795	838	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01839	.	engineered_region	1912	3282	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_S01839	.	ribosome_entry_site	1912	1923	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_S01839	.	conserved_region	1916	1919	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01839	.	terminator	3091	3219	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_S01839	.	terminator	3179	3219	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01839	.	stem_loop	3186	3205	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01839	.	polyA_site	3206	3219	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01839	.	stop_codon	3212	3212	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01839	.	engineered_region	3179	3219	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01839	.	terminator	3091	3170	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01839	.	engineered_region	3091	3170	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01839	.	stem_loop	3102	3145	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01839	.	CDS	1930	3057	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_S01839	.	engineered_tag	3058	3082	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01839	.	engineered_region	1930	3057	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01839	.	tag	3019	3057	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01839	.	CDS	1930	3057	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01839	.	promoter	3228	3281	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_S01839	.	engineered_region	3228	3281	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01839	.	promoter	3270	3275	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01839	.	promoter	3247	3252	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01839	.	operator	3230	3246	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01839	.	operator	3253	3269	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01839	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01839	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S01839
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggg
gactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagct
cttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaag
ggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattga
tgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacct
gtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtag
ccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaa
tccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgta
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaaacaagatacattgtatgaaaa
tacaagaaagtttgttgatactagagaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgc
tcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgg
gagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgc
tgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttg
ggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgta
gattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaag
gaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacga
ggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaa
atcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaact
acgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctt
taatttctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatg
ccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtgga
agcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggc
gttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtg
ccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacg
cgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccg
gcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcg
tggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcg
tctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatg
tccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatgg
cgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatac
cgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggac
cgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaacca
ccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccg
actggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatct
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcgg
ataacaattgacattgtgagcggataacaagatactgagcaca