##gff-version 3
##sequence-region BBa_S01840 1 4795
BBa_S01840	.	promoter	3830	3883	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S01840	.	engineered_region	3830	3883	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01840	.	promoter	3872	3877	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01840	.	promoter	3849	3854	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01840	.	operator	3832	3848	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01840	.	operator	3855	3871	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01840	.	ribosome_entry_site	3893	3904	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01840	.	conserved_region	3897	3900	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01840	.	CDS	3911	4579	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_S01840	.	engineered_region	3911	4579	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_S01840	.	engineered_tag	4580	4604	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_S01840	.	tag	4541	4579	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_S01840	.	CDS	3911	4579	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_S01840	.	promoter	4750	4795	.	+	0	ID=BBa_R0052;Name=BBa_R0052
BBa_S01840	.	promoter	4750	4755	.	+	0	ID=annotation2029;Name=-35;Parent=BBa_R0052
BBa_S01840	.	non_covalent_binding_site	4750	4792	.	+	0	ID=annotation2026;Name=OR2;Parent=BBa_R0052
BBa_S01840	.	promoter	4751	4756	.	+	0	ID=annotation2031;Name=-35;Parent=BBa_R0052
BBa_S01840	.	non_covalent_binding_site	4757	4770	.	+	0	ID=annotation2027;Name=OR2;Parent=BBa_R0052
BBa_S01840	.	start_codon	4784	4786	.	+	0	ID=annotation2032;Name=start;Parent=BBa_R0052
BBa_S01840	.	engineered_region	4750	4795	.	+	0	ID=annotation7068;Name=BBa_R0052;Parent=BBa_R0052
BBa_S01840	.	non_covalent_binding_site	4779	4792	.	+	0	ID=annotation2028;Name=OR1;Parent=BBa_R0052
BBa_S01840	.	promoter	4773	4778	.	+	0	ID=annotation2030;Name=-10;Parent=BBa_R0052
BBa_S01840	.	terminator	4613	4741	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S01840	.	terminator	4701	4741	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01840	.	stem_loop	4708	4727	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01840	.	polyA_site	4728	4741	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01840	.	stop_codon	4734	4734	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01840	.	engineered_region	4701	4741	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01840	.	terminator	4613	4692	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01840	.	stem_loop	4624	4667	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01840	.	engineered_region	4613	4692	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01840	.	engineered_region	1	3821	.	+	0	ID=BBa_I5600;Name=BBa_I5600
BBa_S01840	.	promoter	1933	1986	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_I5600
BBa_S01840	.	non_covalent_binding_site	1933	1935	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_S01840	.	promoter	1962	1967	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_S01840	.	non_covalent_binding_site	1943	1960	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_S01840	.	promoter	1940	1945	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_S01840	.	non_covalent_binding_site	1966	1983	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_S01840	.	engineered_region	1933	1986	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_S01840	.	promoter	1950	1955	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_S01840	.	terminator	3781	3821	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I5600
BBa_S01840	.	stem_loop	3788	3807	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01840	.	polyA_site	3808	3821	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01840	.	stop_codon	3814	3814	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01840	.	engineered_region	3781	3821	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01840	.	promoter	1001	1049	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_I5600
BBa_S01840	.	engineered_region	1001	1049	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S01840	.	promoter	1038	1043	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S01840	.	non_covalent_binding_site	1025	1041	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S01840	.	promoter	1015	1020	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S01840	.	non_covalent_binding_site	1001	1017	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S01840	.	terminator	1796	1875	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I5600
BBa_S01840	.	stem_loop	1807	1850	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01840	.	engineered_region	1796	1875	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01840	.	terminator	2706	2785	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I5600
BBa_S01840	.	stem_loop	2717	2760	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01840	.	engineered_region	2706	2785	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01840	.	ribosome_entry_site	1058	1069	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I5600
BBa_S01840	.	conserved_region	1062	1065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01840	.	CDS	81	830	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I5600
BBa_S01840	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01840	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S01840	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01840	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I5600
BBa_S01840	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01840	.	terminator	3693	3772	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I5600
BBa_S01840	.	stem_loop	3704	3747	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01840	.	engineered_region	3693	3772	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01840	.	ribosome_entry_site	2905	2916	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I5600
BBa_S01840	.	conserved_region	2909	2912	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01840	.	terminator	1884	1924	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I5600
BBa_S01840	.	stem_loop	1891	1910	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01840	.	polyA_site	1911	1924	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01840	.	stop_codon	1917	1917	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01840	.	engineered_region	1884	1924	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01840	.	ribosome_entry_site	1995	2006	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I5600
BBa_S01840	.	conserved_region	1999	2002	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01840	.	CDS	2013	2672	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I5600
BBa_S01840	.	tag	2633	2666	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01840	.	engineered_tag	2673	2697	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01840	.	CDS	2016	2632	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01840	.	terminator	952	992	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I5600
BBa_S01840	.	stem_loop	959	978	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01840	.	polyA_site	979	992	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01840	.	stop_codon	985	985	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01840	.	engineered_region	952	992	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01840	.	terminator	864	943	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I5600
BBa_S01840	.	stem_loop	875	918	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01840	.	engineered_region	864	943	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01840	.	promoter	2843	2896	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I5600
BBa_S01840	.	engineered_region	2843	2896	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01840	.	promoter	2862	2867	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01840	.	non_covalent_binding_site	2868	2886	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01840	.	promoter	2885	2890	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01840	.	non_covalent_binding_site	2843	2861	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01840	.	engineered_region	2923	3684	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_I5600
BBa_S01840	.	tag	3641	3678	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S01840	.	stop_codon	3679	3684	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S01840	.	engineered_region	2923	3684	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S01840	.	point_mutation	2991	2991	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S01840	.	CDS	2923	3684	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S01840	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I5600
BBa_S01840	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01840	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01840	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01840	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01840	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01840	.	terminator	2794	2834	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I5600
BBa_S01840	.	stem_loop	2801	2820	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01840	.	polyA_site	2821	2834	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01840	.	stop_codon	2827	2827	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01840	.	engineered_region	2794	2834	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01840	.	CDS	1076	1762	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_I5600
BBa_S01840	.	stop_codon	1757	1762	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_S01840	.	engineered_tag	1763	1787	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_S01840	.	tag	1724	1756	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_S01840	.	CDS	1076	1723	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_S01840	.	engineered_region	1076	1762	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
>BBa_S01840
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttac
ctctggcggtgataatggttgctactagagaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattc
gcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgca
atgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactat
ttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatca
gttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccac
tgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattcca
gaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaa
acgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgat
agaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaa
aactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgt
tctttaatttctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcatta
gagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacat
tgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactt
ttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcat
cgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttt
tatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtatt
ggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaa
gctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaa
aacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtc
cctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactaga
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatga
agcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcaga
agaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaa
gatactgagcacatactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagc
ttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaac
taagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgat
tcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggt
gtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggatt
ctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagat
actggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactgg
tcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagat
tatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactc
tgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga