LOCUS BBa_S01841 3233 bp DNA linear 25-Nov-2024 DEFINITION --Specify Parts List-- ACCESSION BBa_S01841 VERSION BBa_S01841.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..666 /label=cII p22 misc_feature 667..699 /label=LVA misc_feature 700..705 /label=stop misc_feature 19..705 /label=BBa_C0053 misc_feature 706..730 /label=Help:Barcodes CDS 19..705 /label=BBa_C0053 stem_loop 750..793 /label=stem_loop misc_feature 739..818 /label=BBa_B0010 terminator 739..818 /label=BBa_B0010 stem_loop 834..853 /label=T7 TE misc_feature 860..860 /label=stop polyA_site 854..867 /label=polya misc_feature 827..867 /label=BBa_B0012 terminator 827..867 /label=BBa_B0012 misc_feature 876..878 /label=OR3 misc_feature 886..903 /label=OR2 misc_feature 909..926 /label=OR1 promoter 893..898 /label=-35 promoter 883..888 /label=-35 promoter 905..910 /label=-10 misc_feature 876..929 /label=BBa_R0053 promoter 876..929 /label=BBa_R0053 misc_feature 1..929 /label=BBa_Q04530 misc_feature 942..945 /label=conserved RBS 938..949 /label=BBa_B0034 misc_feature 2045..2083 /label=LVA CDS 956..2083 /label=lacI-LVA misc_feature 956..2083 /label=BBa_C0012 misc_feature 2084..2108 /label=Help:Barcodes CDS 956..2083 /label=BBa_C0012 stem_loop 2128..2171 /label=stem_loop misc_feature 2117..2196 /label=BBa_B0010 terminator 2117..2196 /label=BBa_B0010 stem_loop 2212..2231 /label=T7 TE misc_feature 2238..2238 /label=stop polyA_site 2232..2245 /label=polya misc_feature 2205..2245 /label=BBa_B0012 terminator 2205..2245 /label=BBa_B0012 terminator 2117..2245 /label=BBa_B0015 misc_feature 2256..2272 /label=lac O1 promoter 2273..2278 /label=-35 misc_feature 2279..2295 /label=lac O1 promoter 2296..2301 /label=-10 misc_feature 2254..2307 /label=BBa_R0011 promoter 2254..2307 /label=BBa_R0011 misc_feature 938..2308 /label=BBa_Q04121 misc_feature 2321..2324 /label=conserved RBS 2317..2328 /label=BBa_B0034 CDS 2335..3096 /label=YFP (LVA) misc_feature 3091..3096 /label=2 misc_feature 3052..3090 /label=SsrA misc_feature 2576..2576 /label=A (Q->L) misc_feature 2335..3096 /label=BBa_E0032 misc_feature 2335..3096 /label=BBa_E0032 stem_loop 3116..3159 /label=stem_loop misc_feature 3105..3184 /label=BBa_B0010 terminator 3105..3184 /label=BBa_B0010 stem_loop 3200..3219 /label=T7 TE misc_feature 3226..3226 /label=stop polyA_site 3220..3233 /label=polya misc_feature 3193..3233 /label=BBa_B0012 terminator 3193..3233 /label=BBa_B0012 misc_feature 2317..3233 /label=BBa_E0432 ORIGIN 1 aaagaggaga aatactagat gaatacacaa ttgatgggtg agcgtattcg cgctcgaaga 61 aaaaaactca agattagaca agccgctctt ggtaagatgg tgggagtgtc taatgttgca 121 atatcgcaat gggagcgctc ggagactgag ccaaatgggg agaacctgtt ggcactttcg 181 aaggctcttc agtgctcccc tgactatttg ctgaaaggag atttaagcca gacaaacgtt 241 gcctatcata gtaggcatga gccaagagga tcataccctc ttatcagttg ggtaagcgca 301 gggcaatgga tggaagctgt agaaccttat cacaagcgcg cgatagagaa ctggcacgac 361 accactgtag attgttcaga agattcattt tggcttgatg tccaaggtga ctctatgaca 421 gcaccggcag ggttaagcat tccagaagga atgataattc tggttgatcc cgaagtcgaa 481 ccaagaaacg gcaagctggt tgttgcaaaa ttagaaggtg aaaacgaggc cacattcaaa 541 aaattagtta tggatgcagg ccgaaagttt ttaaaaccat taaacccaca atatccgatg 601 atagaaatca acggaaactg caaaatcatt ggcgtagttg ttgacgcaaa actcgcaaat 661 cttccagctg caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag 721 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 781 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 841 cttcgggtgg gcctttctgc gtttatatac tagagaataa acttgactaa agattccttt 901 agtagataat ttaagtgttc tttaatttct actagagaaa gaggagaaat actagatggt 961 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac 1021 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga 1081 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa 1141 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat 1201 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt 1261 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt 1321 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc 1381 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat 1441 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca 1501 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc 1561 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga 1621 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc 1681 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga 1741 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag 1801 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 1861 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 1921 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 1981 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 2041 gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 2101 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2161 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2221 tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac aattgacatt 2281 gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata ctagatggtg 2341 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 2401 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 2461 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 2521 accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac 2581 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 2641 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 2701 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 2761 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 2821 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 2881 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 2941 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 3001 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct 3061 gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa 3121 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 3181 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //