##gff-version 3
##sequence-region BBa_S01841 1 3233
BBa_S01841	.	engineered_region	2317	3233	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_S01841	.	ribosome_entry_site	2317	2328	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_S01841	.	conserved_region	2321	2324	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01841	.	terminator	3105	3184	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_S01841	.	engineered_region	3105	3184	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01841	.	stem_loop	3116	3159	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01841	.	terminator	3193	3233	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_S01841	.	stem_loop	3200	3219	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01841	.	polyA_site	3220	3233	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01841	.	stop_codon	3226	3226	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01841	.	engineered_region	3193	3233	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01841	.	engineered_region	2335	3096	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_S01841	.	sequence_alteration	2576	2576	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01841	.	engineered_region	2335	3096	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01841	.	tag	3052	3090	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01841	.	CDS	2335	3096	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01841	.	stop_codon	3091	3096	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01841	.	engineered_region	1	929	.	+	0	ID=BBa_Q04530;Name=BBa_Q04530
BBa_S01841	.	promoter	876	929	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_Q04530
BBa_S01841	.	non_covalent_binding_site	876	878	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_S01841	.	promoter	905	910	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_S01841	.	non_covalent_binding_site	886	903	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_S01841	.	promoter	883	888	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_S01841	.	non_covalent_binding_site	909	926	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_S01841	.	engineered_region	876	929	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_S01841	.	promoter	893	898	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_S01841	.	terminator	827	867	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04530
BBa_S01841	.	stem_loop	834	853	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01841	.	polyA_site	854	867	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01841	.	stop_codon	860	860	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01841	.	engineered_region	827	867	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01841	.	CDS	19	705	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_Q04530
BBa_S01841	.	tag	667	699	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_S01841	.	engineered_region	19	705	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_S01841	.	engineered_tag	706	730	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_S01841	.	stop_codon	700	705	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_S01841	.	CDS	19	666	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_S01841	.	terminator	739	818	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04530
BBa_S01841	.	engineered_region	739	818	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01841	.	stem_loop	750	793	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01841	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04530
BBa_S01841	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01841	.	engineered_region	938	2308	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_S01841	.	ribosome_entry_site	938	949	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_S01841	.	conserved_region	942	945	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01841	.	terminator	2117	2245	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_S01841	.	terminator	2205	2245	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01841	.	stem_loop	2212	2231	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01841	.	polyA_site	2232	2245	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01841	.	stop_codon	2238	2238	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01841	.	engineered_region	2205	2245	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01841	.	terminator	2117	2196	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01841	.	engineered_region	2117	2196	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01841	.	stem_loop	2128	2171	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01841	.	CDS	956	2083	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_S01841	.	engineered_tag	2084	2108	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01841	.	engineered_region	956	2083	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01841	.	tag	2045	2083	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01841	.	CDS	956	2083	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01841	.	promoter	2254	2307	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_S01841	.	engineered_region	2254	2307	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01841	.	promoter	2296	2301	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01841	.	promoter	2273	2278	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01841	.	operator	2256	2272	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01841	.	operator	2279	2295	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
>BBa_S01841
aaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagac
aagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatgg
ggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaac
gttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaag
ctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggct
tgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaa
gtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatgg
atgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattgg
cgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactga
tagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaa
atactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcc
cgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattaca
ttcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgca
cgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaa
cgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatc
cgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgacca
gacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcac
cagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctca
ctcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaat
gctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattacc
gagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgc
cgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcca
ggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcc
tctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacg
acgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggata
acaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtg
gtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcca
cctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacctt
cggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaa
ggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcg
acaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagta
caactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccac
aacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgc
ccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgga
gttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgct
ttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata