##gff-version 3
##sequence-region BBa_S01849 1 5441
BBa_S01849	.	terminator	4298	4338	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01849	.	stem_loop	4305	4324	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01849	.	polyA_site	4325	4338	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01849	.	stop_codon	4331	4331	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01849	.	engineered_region	4298	4338	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01849	.	ribosome_entry_site	3541	3552	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01849	.	conserved_region	3545	3548	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01849	.	terminator	5401	5441	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01849	.	stem_loop	5408	5427	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01849	.	polyA_site	5428	5441	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01849	.	stop_codon	5434	5434	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01849	.	engineered_region	5401	5441	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01849	.	terminator	3299	3378	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01849	.	stem_loop	3310	3353	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01849	.	engineered_region	3299	3378	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01849	.	promoter	3436	3532	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S01849	.	promoter	3506	3511	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S01849	.	engineered_region	3436	3532	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S01849	.	promoter	3493	3493	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S01849	.	non_covalent_binding_site	3492	3508	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S01849	.	promoter	3482	3487	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S01849	.	promoter	3529	3532	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S01849	.	non_covalent_binding_site	3516	3532	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S01849	.	non_covalent_binding_site	3441	3460	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S01849	.	promoter	2290	2489	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S01849	.	CDS	2290	2377	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S01849	.	non_covalent_binding_site	2455	2489	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S01849	.	promoter	2450	2455	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S01849	.	promoter	2426	2431	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S01849	.	non_covalent_binding_site	2378	2415	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S01849	.	start_codon	2462	2462	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S01849	.	engineered_region	2290	2489	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S01849	.	terminator	2241	2281	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01849	.	stem_loop	2248	2267	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01849	.	polyA_site	2268	2281	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01849	.	stop_codon	2274	2274	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01849	.	engineered_region	2241	2281	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01849	.	terminator	2153	2232	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01849	.	stem_loop	2164	2207	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01849	.	engineered_region	2153	2232	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01849	.	CDS	2516	3265	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S01849	.	CDS	2519	3226	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01849	.	engineered_tag	3266	3290	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S01849	.	tag	3227	3259	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01849	.	CDS	992	2119	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_S01849	.	engineered_tag	2120	2144	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01849	.	engineered_region	992	2119	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01849	.	tag	2081	2119	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01849	.	CDS	992	2119	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01849	.	ribosome_entry_site	974	985	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01849	.	conserved_region	978	981	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01849	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01849	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01849	.	terminator	4210	4289	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01849	.	stem_loop	4221	4264	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01849	.	engineered_region	4210	4289	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01849	.	terminator	863	903	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01849	.	stem_loop	870	889	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01849	.	polyA_site	890	903	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01849	.	stop_codon	896	896	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01849	.	engineered_region	863	903	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01849	.	CDS	82	741	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01849	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01849	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01849	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01849	.	ribosome_entry_site	2498	2509	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01849	.	conserved_region	2502	2505	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01849	.	terminator	3387	3427	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01849	.	stem_loop	3394	3413	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01849	.	polyA_site	3414	3427	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01849	.	stop_codon	3420	3420	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01849	.	engineered_region	3387	3427	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01849	.	promoter	4347	4497	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_S01849	.	promoter	4461	4468	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S01849	.	non_covalent_binding_site	4476	4495	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S01849	.	non_covalent_binding_site	4347	4366	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S01849	.	start_codon	4474	4474	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S01849	.	engineered_region	4347	4497	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S01849	.	promoter	4435	4440	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S01849	.	CDS	3559	4176	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S01849	.	engineered_tag	4177	4201	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S01849	.	tag	4138	4169	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S01849	.	engineered_region	3559	4176	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S01849	.	CDS	3559	4137	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S01849	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_S01849	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_S01849	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_S01849	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_S01849	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_S01849	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_S01849	.	terminator	775	854	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01849	.	stem_loop	786	829	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01849	.	engineered_region	775	854	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01849	.	terminator	5313	5392	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01849	.	stem_loop	5324	5367	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01849	.	engineered_region	5313	5392	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01849	.	CDS	4524	5279	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S01849	.	engineered_tag	5280	5304	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S01849	.	engineered_region	4524	4525	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S01849	.	point_mutation	4697	4697	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S01849	.	engineered_region	4524	5279	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S01849	.	point_mutation	5015	5015	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S01849	.	CDS	4524	5273	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S01849	.	promoter	912	965	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01849	.	engineered_region	912	965	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01849	.	promoter	931	936	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01849	.	non_covalent_binding_site	937	955	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01849	.	promoter	954	959	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01849	.	non_covalent_binding_site	912	930	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01849	.	ribosome_entry_site	4506	4517	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01849	.	conserved_region	4510	4513	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_S01849
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtc
gcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggg
aaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgtt
gctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgat
caactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttc
tcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcac
taatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacg
cgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcgg
cgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactg
gagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaac
gatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggat
acgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaac
cagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa
agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac
aggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgcta
gtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcg
tatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcacaaaaa
agaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttgg
cttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgca
ttaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagag
aaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgt
tcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaa
gccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctg
acggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatga
gtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatccca
tgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacg
aaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatgg
tttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatac
tagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtt
atcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaa
tgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattat
atgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtt
ttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataa
acacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaa
gttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctatta
atgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtg
ctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gacctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaattta
ttagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactag
agaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtag
aagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatt
tatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacg
ctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaa
caatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccct
attcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttt
tacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataa
atcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttca
aaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgct
gccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata