LOCUS       BBa_S01850              2164 bp    DNA     linear       22-Dec-2024
DEFINITION  Intermediate part from assembly 240
ACCESSION   BBa_S01850
VERSION     BBa_S01850.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    1010..1015
                     /label=2
     misc_feature    977..1009
                     /label=LVA
     CDS             227..976
                     /label=aiiA
     misc_feature    227..1015
                     /label=BBa_C0060
     misc_feature    1016..1040
                     /label=Help:Barcodes
     CDS             227..1015
                     /label=BBa_C0060
     stem_loop       1060..1103
                     /label=stem_loop
     misc_feature    1049..1128
                     /label=BBa_B0010
     terminator      1049..1128
                     /label=BBa_B0010
     stem_loop       1144..1163
                     /label=T7 TE
     misc_feature    1170..1170
                     /label=stop
     polyA_site      1164..1177
                     /label=polya
     misc_feature    1137..1177
                     /label=BBa_B0012
     terminator      1137..1177
                     /label=BBa_B0012
     misc_feature    1186..1204
                     /label=TetR 1
     misc_feature    1186..1239
                     /label=BBa_R0040
     promoter        1205..1210
                     /label=-35
     misc_feature    1211..1229
                     /label=TetR 2
     promoter        1228..1233
                     /label=-10
     promoter        1186..1239
                     /label=BBa_R0040
     misc_feature    1252..1255
                     /label=conserved
     RBS             1248..1259
                     /label=BBa_B0034
     CDS             1266..2027
                     /label=YFP (LVA)
     misc_feature    2022..2027
                     /label=2
     misc_feature    1983..2021
                     /label=SsrA
     misc_feature    1507..1507
                     /label=A (Q->L)
     misc_feature    1266..2027
                     /label=BBa_E0032
     misc_feature    1266..2027
                     /label=BBa_E0032
     stem_loop       2047..2090
                     /label=stem_loop
     misc_feature    2036..2115
                     /label=BBa_B0010
     terminator      2036..2115
                     /label=BBa_B0010
     stem_loop       2131..2150
                     /label=T7 TE
     misc_feature    2157..2157
                     /label=stop
     polyA_site      2151..2164
                     /label=polya
     misc_feature    2124..2164
                     /label=BBa_B0012
     terminator      2124..2164
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga cagtaaagaa
      241 gctttatttc gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt
      301 aacaccagga gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg
      361 acctatttta gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa
      421 cggtacattt gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa
      481 tattttaaaa cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt
      541 gcattttgat catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg
      601 tgctgaatat gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa
      661 tttaaactac aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca
      721 tacaccaggc catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc
      781 tgtattatta acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt
      841 tgcgggattt gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat
      901 gaaagagaag ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt
      961 gttccctgaa tatatagctg caaacgacga aaactacgct ttagtagctt aataacgctg
     1021 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1081 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1141 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata
     1201 gagattgaca tccctatcag tgatagagat actgagcact actagagaaa gaggagaaat
     1261 actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1321 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1381 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1441 ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca
     1501 tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1561 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1621 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1681 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1741 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1801 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1861 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1921 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1981 agaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg
     2041 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2101 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2161 tata
//