##gff-version 3
##sequence-region BBa_S01851 1 3337
BBa_S01851	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01851	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01851	.	promoter	3284	3337	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01851	.	engineered_region	3284	3337	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01851	.	promoter	3303	3308	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01851	.	non_covalent_binding_site	3309	3327	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01851	.	promoter	3326	3331	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01851	.	non_covalent_binding_site	3284	3302	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01851	.	terminator	952	992	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01851	.	stem_loop	959	978	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01851	.	polyA_site	979	992	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01851	.	stop_codon	985	985	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01851	.	engineered_region	952	992	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01851	.	terminator	3147	3226	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01851	.	stem_loop	3158	3201	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01851	.	engineered_region	3147	3226	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01851	.	terminator	864	943	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01851	.	stem_loop	875	918	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01851	.	engineered_region	864	943	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01851	.	terminator	2237	2316	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01851	.	stem_loop	2248	2291	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01851	.	engineered_region	2237	2316	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01851	.	ribosome_entry_site	2436	2447	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01851	.	conserved_region	2440	2443	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01851	.	ribosome_entry_site	1058	1069	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01851	.	conserved_region	1062	1065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01851	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01851	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01851	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01851	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01851	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01851	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01851	.	CDS	2454	3113	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01851	.	tag	3074	3107	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01851	.	engineered_tag	3114	3138	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01851	.	CDS	2457	3073	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01851	.	promoter	2374	2427	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S01851	.	engineered_region	2374	2427	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01851	.	promoter	2416	2421	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01851	.	promoter	2393	2398	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01851	.	operator	2376	2392	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01851	.	operator	2399	2415	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01851	.	promoter	1001	1049	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_S01851	.	engineered_region	1001	1049	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_S01851	.	promoter	1038	1043	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_S01851	.	non_covalent_binding_site	1025	1041	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_S01851	.	promoter	1015	1020	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_S01851	.	non_covalent_binding_site	1001	1017	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_S01851	.	CDS	81	830	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_S01851	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01851	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S01851	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01851	.	CDS	1076	2203	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_S01851	.	engineered_tag	2204	2228	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01851	.	engineered_region	1076	2203	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01851	.	tag	2165	2203	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01851	.	CDS	1076	2203	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01851	.	terminator	3235	3275	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01851	.	stem_loop	3242	3261	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01851	.	polyA_site	3262	3275	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01851	.	stop_codon	3268	3268	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01851	.	engineered_region	3235	3275	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01851	.	terminator	2325	2365	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01851	.	stem_loop	2332	2351	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01851	.	polyA_site	2352	2365	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01851	.	stop_codon	2358	2358	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01851	.	engineered_region	2325	2365	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S01851
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttac
ctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacg
atgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaac
gcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacag
tcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcg
ccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaa
tcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcc
tgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacg
gtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgt
ctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggc
gactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttg
ccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagt
gggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctgggg
caaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactgg
tgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc
acgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatag
tgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcactactagagaaagaggagaaata
ctagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttta
acaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgc
tcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattt
tttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacgg
cctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcat
tatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaaga
aagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagag
ccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaa
acgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcac