##gff-version 3
##sequence-region BBa_S01889 1 4263
BBa_S01889	.	CDS	3380	4039	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01889	.	CDS	3383	3999	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01889	.	tag	4000	4033	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01889	.	engineered_tag	4040	4064	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01889	.	ribosome_entry_site	3362	3373	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01889	.	conserved_region	3366	3369	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01889	.	terminator	2095	2135	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01889	.	polyA_site	2122	2135	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01889	.	engineered_region	2095	2135	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01889	.	stem_loop	2102	2121	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01889	.	stop_codon	2128	2128	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01889	.	engineered_region	1237	1998	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_S01889	.	CDS	1237	1998	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_S01889	.	tag	1955	1992	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_S01889	.	point_mutation	1305	1305	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_S01889	.	stop_codon	1993	1998	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_S01889	.	engineered_region	1237	1998	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_S01889	.	promoter	2144	3353	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_S01889	.	promoter	3186	3257	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_S01889	.	sequence_feature	2144	3222	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_S01889	.	promoter	3223	3353	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_S01889	.	terminator	2007	2086	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01889	.	engineered_region	2007	2086	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01889	.	stem_loop	2018	2061	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01889	.	terminator	4161	4201	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_S01889	.	polyA_site	4188	4201	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01889	.	engineered_region	4161	4201	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01889	.	stem_loop	4168	4187	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01889	.	stop_codon	4194	4194	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01889	.	promoter	4210	4263	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01889	.	engineered_region	4210	4263	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01889	.	non_covalent_binding_site	4210	4228	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01889	.	promoter	4229	4234	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01889	.	promoter	4252	4257	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01889	.	non_covalent_binding_site	4235	4253	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01889	.	terminator	4073	4152	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S01889	.	engineered_region	4073	4152	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01889	.	stem_loop	4084	4127	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01889	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01889	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01889	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_S01889	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_S01889	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_S01889	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
>BBa_S01889
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttca
ccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaggg
cgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg
accaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcca
tgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagtt
cgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag
ctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaaga
tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt
gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtc
ctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaa
actacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggc
cccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggc
atccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaact
gctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtc
tgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgct
tccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgt
taatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatat
tgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctcc
ggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccct
gatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaa
atcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggga
tcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacatt
gccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaag
cgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcac
ggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactct
ctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac