LOCUS       BBa_S01905              2183 bp    DNA     linear       10-May-2024
DEFINITION  Intermediate part from assembly 240
ACCESSION   BBa_S01905
VERSION     BBa_S01905.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     CDS             945..1032
                     /label=end of LacI coding region (inactive)
     misc_feature    945..1144
                     /label=BBa_R0010
     misc_feature    1033..1070
                     /label=CAP binding site
     promoter        1081..1086
                     /label=-35
     promoter        1105..1110
                     /label=-10
     misc_feature    1110..1144
                     /label=LacI binding site
     misc_feature    1117..1117
                     /label=start
     promoter        945..1144
                     /label=BBa_R0010
     misc_feature    1157..1160
                     /label=conserved
     RBS             1153..1164
                     /label=BBa_B0034
     misc_feature    1171..1173
                     /label=start
     misc_feature    1954..1959
                     /label=2
     misc_feature    1921..1953
                     /label=LVA
     CDS             1171..1920
                     /label=aiiA
     misc_feature    1171..1959
                     /label=BBa_C0060
     misc_feature    1960..1984
                     /label=Help:Barcodes
     CDS             1171..1959
                     /label=BBa_C0060
     stem_loop       2004..2047
                     /label=stem_loop
     misc_feature    1993..2072
                     /label=BBa_B0010
     terminator      1993..2072
                     /label=BBa_B0010
     stem_loop       2088..2107
                     /label=T7 TE
     misc_feature    2114..2114
                     /label=stop
     polyA_site      2108..2121
                     /label=polya
     misc_feature    2081..2121
                     /label=BBa_B0012
     terminator      2081..2121
                     /label=BBa_B0012
     misc_feature    2130..2148
                     /label=TetR 1
     misc_feature    2130..2183
                     /label=BBa_R0040
     promoter        2149..2154
                     /label=-35
     misc_feature    2155..2173
                     /label=TetR 2
     promoter        2172..2177
                     /label=-10
     promoter        2130..2183
                     /label=BBa_R0040
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagcaatac gcaaaccgcc
      961 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1021 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     1081 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     1141 cacatactag agaaagagga gaaatactag atgacagtaa agaagcttta tttcgtccca
     1201 gcaggtcgtt gtatgttgga tcattcgtct gttaatagta cattaacacc aggagaatta
     1261 ttagacttac cggtttggtg ttatcttttg gagactgaag aaggacctat tttagtagat
     1321 acaggtatgc cagaaagtgc agttaataat gaaggtcttt ttaacggtac atttgtcgaa
     1381 gggcaggttt taccgaaaat gactgaagaa gatagaatcg tgaatatttt aaaacgggtt
     1441 ggttatgagc cggaagacct tctttatatt attagttctc acttgcattt tgatcatgca
     1501 ggaggaaatg gcgcttttat aaatacacca atcattgtac agcgtgctga atatgaggcg
     1561 gcgcagcata gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc
     1621 attgaaggtg attatgaagt cgtaccagga gttcaattat tgcatacacc aggccatact
     1681 ccagggcatc aatcgctatt aattgagaca gaaaaatccg gtcctgtatt attaacgatt
     1741 gatgcatcgt atacgaaaga gaattttgaa aatgaagtgc catttgcggg atttgattca
     1801 gaattagctt tatcttcaat taaacgttta aaagaagtgg tgatgaaaga gaagccgatt
     1861 gttttctttg gacatgatat agagcaggaa aggggatgta aagtgttccc tgaatatata
     1921 gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga
     1981 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2041 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2101 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta
     2161 tcagtgatag agatactgag cac
//