LOCUS       BBa_S01927               899 bp    DNA     linear       22-Nov-2024
DEFINITION  Intermediate part from assembly 283
ACCESSION   BBa_S01927
VERSION     BBa_S01927.1  
FEATURES             Location/Qualifiers
     CDS             1..762
                     /label=YFP (LVA)
     misc_feature    757..762
                     /label=2
     misc_feature    718..756
                     /label=SsrA
     misc_feature    242..242
                     /label=A (Q->L)
     misc_feature    1..762
                     /label=BBa_E0032
     misc_feature    1..762
                     /label=BBa_E0032
     stem_loop       782..825
                     /label=stem_loop
     misc_feature    771..850
                     /label=BBa_B0010
     terminator      771..850
                     /label=BBa_B0010
     stem_loop       866..885
                     /label=T7 TE
     misc_feature    892..892
                     /label=stop
     polyA_site      886..899
                     /label=polya
     misc_feature    859..899
                     /label=BBa_B0012
     terminator      859..899
                     /label=BBa_B0012
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag
      241 ctgcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg
      721 cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca
      781 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      841 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//
LOCUS       BBa_I9045                899 bp    DNA     linear       22-Nov-2024
DEFINITION  reporter (yfp for cin) E0032.B0015
ACCESSION   BBa_I9045
VERSION     BBa_I9045.1  
FEATURES             Location/Qualifiers
     CDS             1..762
                     /label=YFP (LVA)
     misc_feature    757..762
                     /label=2
     misc_feature    718..756
                     /label=SsrA
     misc_feature    242..242
                     /label=A (Q->L)
     misc_feature    1..762
                     /label=BBa_E0032
     misc_feature    1..762
                     /label=BBa_E0032
     stem_loop       782..825
                     /label=stem_loop
     misc_feature    771..850
                     /label=BBa_B0010
     terminator      771..850
                     /label=BBa_B0010
     stem_loop       866..885
                     /label=T7 TE
     misc_feature    892..892
                     /label=stop
     polyA_site      886..899
                     /label=polya
     misc_feature    859..899
                     /label=BBa_B0012
     terminator      859..899
                     /label=BBa_B0012
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag
      241 ctgcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg
      721 cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca
      781 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      841 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//