LOCUS BBa_S01932 3108 bp DNA linear 25-Nov-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01932 VERSION BBa_S01932.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 19..20 /label=prefix misc_feature 192..192 /label=T misc_feature 510..510 /label=A CDS 19..768 /label=luxR misc_feature 19..774 /label=BBa_C0062 misc_feature 775..799 /label=Help:Barcodes CDS 19..774 /label=BBa_C0062 stem_loop 819..862 /label=stem_loop misc_feature 808..887 /label=BBa_B0010 terminator 808..887 /label=BBa_B0010 stem_loop 903..922 /label=T7 TE misc_feature 929..929 /label=stop polyA_site 923..936 /label=polya misc_feature 896..936 /label=BBa_B0012 terminator 896..936 /label=BBa_B0012 CDS 945..1032 /label=end of LacI coding region (inactive) misc_feature 945..1144 /label=BBa_R0010 misc_feature 1033..1070 /label=CAP binding site promoter 1081..1086 /label=-35 promoter 1105..1110 /label=-10 misc_feature 1110..1144 /label=LacI binding site misc_feature 1117..1117 /label=start promoter 945..1144 /label=BBa_R0010 misc_feature 1157..1160 /label=conserved RBS 1153..1164 /label=BBa_B0034 misc_feature 1171..1173 /label=start misc_feature 1954..1959 /label=2 misc_feature 1921..1953 /label=LVA CDS 1171..1920 /label=aiiA misc_feature 1171..1959 /label=BBa_C0060 misc_feature 1960..1984 /label=Help:Barcodes CDS 1171..1959 /label=BBa_C0060 stem_loop 2004..2047 /label=stem_loop misc_feature 1993..2072 /label=BBa_B0010 terminator 1993..2072 /label=BBa_B0010 stem_loop 2088..2107 /label=T7 TE misc_feature 2114..2114 /label=stop polyA_site 2108..2121 /label=polya misc_feature 2081..2121 /label=BBa_B0012 terminator 2081..2121 /label=BBa_B0012 misc_feature 2130..2148 /label=TetR 1 misc_feature 2130..2183 /label=BBa_R0040 promoter 2149..2154 /label=-35 misc_feature 2155..2173 /label=TetR 2 promoter 2172..2177 /label=-10 promoter 2130..2183 /label=BBa_R0040 misc_feature 2196..2199 /label=conserved RBS 2192..2203 /label=BBa_B0034 CDS 2210..2971 /label=YFP (LVA) misc_feature 2966..2971 /label=2 misc_feature 2927..2965 /label=SsrA misc_feature 2451..2451 /label=A (Q->L) misc_feature 2210..2971 /label=BBa_E0032 misc_feature 2210..2971 /label=BBa_E0032 stem_loop 2991..3034 /label=stem_loop misc_feature 2980..3059 /label=BBa_B0010 terminator 2980..3059 /label=BBa_B0010 stem_loop 3075..3094 /label=T7 TE misc_feature 3101..3101 /label=stop polyA_site 3095..3108 /label=polya misc_feature 3068..3108 /label=BBa_B0012 terminator 3068..3108 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat 61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa 121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct 181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat 241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg 301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa 361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga 421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg 481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata 541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca 601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc 661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt 721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga 781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagcaatac gcaaaccgcc 961 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 1021 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 1081 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 1141 cacatactag agaaagagga gaaatactag atgacagtaa agaagcttta tttcgtccca 1201 gcaggtcgtt gtatgttgga tcattcgtct gttaatagta cattaacacc aggagaatta 1261 ttagacttac cggtttggtg ttatcttttg gagactgaag aaggacctat tttagtagat 1321 acaggtatgc cagaaagtgc agttaataat gaaggtcttt ttaacggtac atttgtcgaa 1381 gggcaggttt taccgaaaat gactgaagaa gatagaatcg tgaatatttt aaaacgggtt 1441 ggttatgagc cggaagacct tctttatatt attagttctc acttgcattt tgatcatgca 1501 ggaggaaatg gcgcttttat aaatacacca atcattgtac agcgtgctga atatgaggcg 1561 gcgcagcata gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc 1621 attgaaggtg attatgaagt cgtaccagga gttcaattat tgcatacacc aggccatact 1681 ccagggcatc aatcgctatt aattgagaca gaaaaatccg gtcctgtatt attaacgatt 1741 gatgcatcgt atacgaaaga gaattttgaa aatgaagtgc catttgcggg atttgattca 1801 gaattagctt tatcttcaat taaacgttta aaagaagtgg tgatgaaaga gaagccgatt 1861 gttttctttg gacatgatat agagcaggaa aggggatgta aagtgttccc tgaatatata 1921 gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga 1981 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2041 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2101 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta 2161 tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga tggtgagcaa 2221 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 2281 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 2341 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 2401 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt 2461 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 2521 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 2581 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 2641 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 2701 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 2761 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 2821 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 2881 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa 2941 cgacgaaaac tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa 3001 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3061 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //