LOCUS BBa_S01984 2945 bp DNA linear 25-Nov-2024 DEFINITION --Specify Parts List-- ACCESSION BBa_S01984 VERSION BBa_S01984.1 FEATURES Location/Qualifiers misc_feature 1..2 /label=prefix misc_feature 174..174 /label=T misc_feature 492..492 /label=A CDS 1..750 /label=luxR misc_feature 1..756 /label=BBa_C0062 misc_feature 757..781 /label=Help:Barcodes CDS 1..756 /label=BBa_C0062 stem_loop 801..844 /label=stem_loop misc_feature 790..869 /label=BBa_B0010 terminator 790..869 /label=BBa_B0010 stem_loop 885..904 /label=T7 TE misc_feature 911..911 /label=stop polyA_site 905..918 /label=polya misc_feature 878..918 /label=BBa_B0012 terminator 878..918 /label=BBa_B0012 terminator 790..918 /label=BBa_B0015 misc_feature 929..945 /label=lac O1 promoter 946..951 /label=-35 misc_feature 952..968 /label=lac O1 promoter 969..974 /label=-10 misc_feature 927..980 /label=BBa_R0011 promoter 927..980 /label=BBa_R0011 misc_feature 994..997 /label=conserved RBS 990..1001 /label=BBa_B0034 misc_feature 1008..1010 /label=start misc_feature 1791..1796 /label=2 misc_feature 1758..1790 /label=LVA CDS 1008..1757 /label=aiiA misc_feature 1008..1796 /label=BBa_C0060 misc_feature 1797..1821 /label=Help:Barcodes CDS 1008..1796 /label=BBa_C0060 stem_loop 1841..1884 /label=stem_loop misc_feature 1830..1909 /label=BBa_B0010 terminator 1830..1909 /label=BBa_B0010 stem_loop 1925..1944 /label=T7 TE misc_feature 1951..1951 /label=stop polyA_site 1945..1958 /label=polya misc_feature 1918..1958 /label=BBa_B0012 terminator 1918..1958 /label=BBa_B0012 misc_feature 990..1958 /label=BBa_I0460 misc_feature 1967..1985 /label=TetR 1 misc_feature 1967..2020 /label=BBa_R0040 promoter 1986..1991 /label=-35 misc_feature 1992..2010 /label=TetR 2 promoter 2009..2014 /label=-10 promoter 1967..2020 /label=BBa_R0040 misc_feature 2033..2036 /label=conserved RBS 2029..2040 /label=BBa_B0034 CDS 2047..2808 /label=YFP (LVA) misc_feature 2803..2808 /label=2 misc_feature 2764..2802 /label=SsrA misc_feature 2288..2288 /label=A (Q->L) misc_feature 2047..2808 /label=BBa_E0032 misc_feature 2047..2808 /label=BBa_E0032 stem_loop 2828..2871 /label=stem_loop misc_feature 2817..2896 /label=BBa_B0010 terminator 2817..2896 /label=BBa_B0010 stem_loop 2912..2931 /label=T7 TE misc_feature 2938..2938 /label=stop polyA_site 2932..2945 /label=polya misc_feature 2905..2945 /label=BBa_B0012 terminator 2905..2945 /label=BBa_B0012 misc_feature 2029..2945 /label=BBa_E0432 ORIGIN 1 atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga 61 agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat 121 tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat 181 aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct 241 atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat 301 gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact 361 gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat 421 tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta 481 attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac 541 aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct 601 tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat 661 gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca 721 ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca 781 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 901 ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac attgtgagcg 961 gataacaaga tactgagcac atactagaga aagaggagaa atactagatg acagtaaaga 1021 agctttattt cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt aatagtacat 1081 taacaccagg agaattatta gacttaccgg tttggtgtta tcttttggag actgaagaag 1141 gacctatttt agtagataca ggtatgccag aaagtgcagt taataatgaa ggtcttttta 1201 acggtacatt tgtcgaaggg caggttttac cgaaaatgac tgaagaagat agaatcgtga 1261 atattttaaa acgggttggt tatgagccgg aagaccttct ttatattatt agttctcact 1321 tgcattttga tcatgcagga ggaaatggcg cttttataaa tacaccaatc attgtacagc 1381 gtgctgaata tgaggcggcg cagcatagcg aagaatattt gaaagaatgt atattgccga 1441 atttaaacta caaaatcatt gaaggtgatt atgaagtcgt accaggagtt caattattgc 1501 atacaccagg ccatactcca gggcatcaat cgctattaat tgagacagaa aaatccggtc 1561 ctgtattatt aacgattgat gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat 1621 ttgcgggatt tgattcagaa ttagctttat cttcaattaa acgtttaaaa gaagtggtga 1681 tgaaagagaa gccgattgtt ttctttggac atgatataga gcaggaaagg ggatgtaaag 1741 tgttccctga atatatagct gcaaacgacg aaaactacgc tttagtagct taataacgct 1801 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc 1861 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1921 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat 1981 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa 2041 tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2101 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 2161 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2221 cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac 2281 atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2341 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 2401 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2461 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 2521 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 2581 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 2641 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 2701 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 2761 aagaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag 2821 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2881 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2941 ttata //