##gff-version 3
##sequence-region BBa_S01984 1 2945
BBa_S01984	.	engineered_region	2029	2945	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_S01984	.	terminator	2817	2896	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_S01984	.	stem_loop	2828	2871	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01984	.	engineered_region	2817	2896	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01984	.	ribosome_entry_site	2029	2040	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_S01984	.	conserved_region	2033	2036	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01984	.	engineered_region	2047	2808	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_S01984	.	CDS	2047	2808	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_S01984	.	tag	2764	2802	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_S01984	.	engineered_region	2047	2808	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_S01984	.	stop_codon	2803	2808	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_S01984	.	sequence_alteration	2288	2288	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_S01984	.	terminator	2905	2945	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_S01984	.	engineered_region	2905	2945	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01984	.	stop_codon	2938	2938	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01984	.	stem_loop	2912	2931	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01984	.	polyA_site	2932	2945	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01984	.	CDS	1	756	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_S01984	.	CDS	1	750	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S01984	.	engineered_region	1	756	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S01984	.	engineered_tag	757	781	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S01984	.	point_mutation	174	174	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S01984	.	engineered_region	1	2	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S01984	.	point_mutation	492	492	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S01984	.	promoter	1967	2020	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01984	.	promoter	1986	1991	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01984	.	non_covalent_binding_site	1992	2010	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01984	.	engineered_region	1967	2020	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01984	.	non_covalent_binding_site	1967	1985	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01984	.	promoter	2009	2014	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01984	.	promoter	927	980	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_S01984	.	promoter	946	951	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01984	.	promoter	969	974	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01984	.	operator	929	945	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01984	.	operator	952	968	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01984	.	engineered_region	927	980	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01984	.	engineered_region	990	1958	.	+	0	ID=BBa_I0460;Name=BBa_I0460
BBa_S01984	.	ribosome_entry_site	990	1001	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_S01984	.	conserved_region	994	997	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01984	.	terminator	1830	1909	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_S01984	.	stem_loop	1841	1884	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01984	.	engineered_region	1830	1909	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01984	.	terminator	1918	1958	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_S01984	.	engineered_region	1918	1958	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01984	.	stop_codon	1951	1951	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01984	.	stem_loop	1925	1944	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01984	.	polyA_site	1945	1958	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01984	.	CDS	1008	1796	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_S01984	.	stop_codon	1791	1796	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_S01984	.	start_codon	1008	1010	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_S01984	.	engineered_region	1008	1796	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_S01984	.	engineered_tag	1797	1821	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_S01984	.	tag	1758	1790	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_S01984	.	CDS	1008	1757	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_S01984	.	terminator	790	918	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S01984	.	terminator	790	869	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01984	.	stem_loop	801	844	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01984	.	engineered_region	790	869	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01984	.	terminator	878	918	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01984	.	engineered_region	878	918	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01984	.	stop_codon	911	911	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01984	.	stem_loop	885	904	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01984	.	polyA_site	905	918	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_S01984
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatc
aatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaa
atctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgat
cctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaat
ctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatgg
cttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacata
ccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacca
aaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtga
gcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagca
attttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattg
acattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgacagtaaagaagcttta
tttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagactta
ccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaata
atgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatat
tttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggagga
aatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttga
aagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgca
tacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgat
gcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaatta
aacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaa
agtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtaga
tcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatca
gtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgag
caagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagc
gtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccg
tgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgca
cgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaag
acccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacg
gcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacgg
catcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacc
cccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacg
agaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagag
gcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata