##gff-version 3
##sequence-region BBa_S01986 1 4414
BBa_S01986	.	terminator	4107	4235	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S01986	.	terminator	4195	4235	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01986	.	polyA_site	4222	4235	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01986	.	engineered_region	4195	4235	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01986	.	stem_loop	4202	4221	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01986	.	stop_codon	4228	4228	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01986	.	terminator	4107	4186	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01986	.	stem_loop	4118	4161	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01986	.	engineered_region	4107	4186	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01986	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_S01986	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S01986	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S01986	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S01986	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S01986	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S01986	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S01986	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S01986	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S01986	.	promoter	954	1007	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_S01986	.	engineered_region	954	1007	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S01986	.	non_covalent_binding_site	954	972	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S01986	.	non_covalent_binding_site	979	997	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S01986	.	promoter	996	1001	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S01986	.	promoter	973	978	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S01986	.	engineered_region	2395	3429	.	+	0	ID=BBa_Q04511;Name=BBa_Q04511
BBa_S01986	.	CDS	2413	3162	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04511
BBa_S01986	.	CDS	2416	3123	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_S01986	.	tag	3124	3156	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_S01986	.	engineered_tag	3163	3187	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_S01986	.	promoter	3333	3429	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_Q04511
BBa_S01986	.	promoter	3426	3429	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_S01986	.	promoter	3403	3408	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_S01986	.	promoter	3390	3390	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_S01986	.	promoter	3379	3384	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_S01986	.	engineered_region	3333	3429	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_S01986	.	non_covalent_binding_site	3413	3429	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_S01986	.	non_covalent_binding_site	3389	3405	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_S01986	.	non_covalent_binding_site	3338	3357	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_S01986	.	terminator	3196	3275	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04511
BBa_S01986	.	stem_loop	3207	3250	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01986	.	engineered_region	3196	3275	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01986	.	terminator	3284	3324	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04511
BBa_S01986	.	polyA_site	3311	3324	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01986	.	engineered_region	3284	3324	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01986	.	stem_loop	3291	3310	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01986	.	stop_codon	3317	3317	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01986	.	ribosome_entry_site	2395	2406	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04511
BBa_S01986	.	conserved_region	2399	2402	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01986	.	CDS	3456	4073	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_S01986	.	engineered_region	3456	4073	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S01986	.	tag	4035	4066	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_S01986	.	engineered_tag	4074	4098	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S01986	.	CDS	3456	4034	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S01986	.	engineered_region	1016	2386	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_S01986	.	ribosome_entry_site	1016	1027	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_S01986	.	conserved_region	1020	1023	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01986	.	terminator	2195	2323	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_S01986	.	terminator	2283	2323	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01986	.	polyA_site	2310	2323	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01986	.	engineered_region	2283	2323	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01986	.	stem_loop	2290	2309	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01986	.	stop_codon	2316	2316	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01986	.	terminator	2195	2274	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01986	.	stem_loop	2206	2249	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01986	.	engineered_region	2195	2274	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S01986	.	CDS	1034	2161	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_S01986	.	engineered_region	1034	2161	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S01986	.	engineered_tag	2162	2186	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S01986	.	tag	2123	2161	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S01986	.	CDS	1034	2161	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S01986	.	promoter	2332	2385	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_S01986	.	promoter	2351	2356	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S01986	.	operator	2357	2373	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S01986	.	engineered_region	2332	2385	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S01986	.	promoter	2374	2379	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S01986	.	operator	2334	2350	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S01986	.	ribosome_entry_site	4403	4414	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01986	.	conserved_region	4407	4410	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01986	.	CDS	124	808	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_S01986	.	tag	744	777	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S01986	.	engineered_tag	784	808	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S01986	.	CDS	127	743	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S01986	.	promoter	4244	4394	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_S01986	.	start_codon	4371	4371	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_S01986	.	engineered_region	4244	4394	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_S01986	.	promoter	4332	4337	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_S01986	.	non_covalent_binding_site	4244	4263	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_S01986	.	non_covalent_binding_site	4373	4392	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_S01986	.	promoter	4358	4365	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_S01986	.	ribosome_entry_site	3438	3449	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01986	.	conserved_region	3442	3445	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01986	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S01986	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S01986	.	terminator	817	945	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_S01986	.	terminator	905	945	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S01986	.	polyA_site	932	945	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S01986	.	engineered_region	905	945	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S01986	.	stem_loop	912	931	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S01986	.	stop_codon	938	938	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S01986	.	terminator	817	896	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S01986	.	stem_loop	828	871	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S01986	.	engineered_region	817	896	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S01986
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacag
cgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcag
cctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccata
ctcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttact
aagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatta
gcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggtt
gcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattatt
acgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgga
ttagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgat
agtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatac
tagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
tactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttccc
gcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacat
tcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcac
gcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaac
gaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatcc
gctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccag
acacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcacc
agcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcac
tcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatg
ctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccg
agtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgcc
gttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccag
gcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcct
ctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacga
cgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataa
caagatactgagcacatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagc
ttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcaga
caagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattg
cttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcgg
ttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctga
gcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggctt
gaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgacc
ctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcag
ggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtg
gggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgt
gcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagatgactataatgataaaaaaa
tcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttg
agtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtga
tgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaa
ttgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaa
agataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattac
agaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagac
aaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtct
taaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgct
tatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccatta
ccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaa