LOCUS BBa_S01997 1901 bp DNA linear 23-Dec-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01997 VERSION BBa_S01997.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 stem_loop 681..724 /label=stem_loop misc_feature 670..749 /label=BBa_B0010 terminator 670..749 /label=BBa_B0010 stem_loop 765..784 /label=T7 TE misc_feature 791..791 /label=stop polyA_site 785..798 /label=polya misc_feature 758..798 /label=BBa_B0012 terminator 758..798 /label=BBa_B0012 misc_feature 807..826 /label=LuxR/HSL promoter 921..928 /label=-10 promoter 895..900 /label=-35 misc_feature 934..934 /label=start misc_feature 936..955 /label=Putative LuxR/HSL misc_feature 807..957 /label=BBa_R0063 promoter 807..957 /label=BBa_R0063 misc_feature 970..973 /label=conserved RBS 966..977 /label=BBa_B0034 misc_feature 984..985 /label=prefix misc_feature 1157..1157 /label=T misc_feature 1475..1475 /label=A CDS 984..1733 /label=luxR misc_feature 984..1739 /label=BBa_C0062 misc_feature 1740..1764 /label=Help:Barcodes CDS 984..1739 /label=BBa_C0062 stem_loop 1784..1827 /label=stem_loop misc_feature 1773..1852 /label=BBa_B0010 terminator 1773..1852 /label=BBa_B0010 stem_loop 1868..1887 /label=T7 TE misc_feature 1894..1894 /label=stop polyA_site 1888..1901 /label=polya misc_feature 1861..1901 /label=BBa_B0012 terminator 1861..1901 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 721 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 781 ggcctttctg cgtttatata ctagagacct gtacgatcct acaggtgctt atgttaagta 841 attgtattcc cagcgataca atagtgtgac aaaaatccaa tttattagaa tcaaatgtca 901 atccattacc gttttaatga tatataacac gcaaaacttg cgacaaacaa taggtaatac 961 tagagaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa 1021 ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga 1081 ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta 1141 aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg 1201 ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta 1261 attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag 1321 cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct 1381 tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac 1441 atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa 1501 atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt 1561 gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta 1621 ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa 1681 gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac 1741 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 1801 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1861 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a // LOCUS BBa_S01818 1901 bp DNA linear 23-Dec-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01818 VERSION BBa_S01818.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 stem_loop 681..724 /label=stem_loop misc_feature 670..749 /label=BBa_B0010 terminator 670..749 /label=BBa_B0010 stem_loop 765..784 /label=T7 TE misc_feature 791..791 /label=stop polyA_site 785..798 /label=polya misc_feature 758..798 /label=BBa_B0012 terminator 758..798 /label=BBa_B0012 misc_feature 807..826 /label=LuxR/HSL promoter 921..928 /label=-10 promoter 895..900 /label=-35 misc_feature 934..934 /label=start misc_feature 936..955 /label=Putative LuxR/HSL misc_feature 807..957 /label=BBa_R0063 promoter 807..957 /label=BBa_R0063 misc_feature 970..973 /label=conserved RBS 966..977 /label=BBa_B0034 misc_feature 984..985 /label=prefix misc_feature 1157..1157 /label=T misc_feature 1475..1475 /label=A CDS 984..1733 /label=luxR misc_feature 984..1739 /label=BBa_C0062 misc_feature 1740..1764 /label=Help:Barcodes CDS 984..1739 /label=BBa_C0062 stem_loop 1784..1827 /label=stem_loop misc_feature 1773..1852 /label=BBa_B0010 terminator 1773..1852 /label=BBa_B0010 stem_loop 1868..1887 /label=T7 TE misc_feature 1894..1894 /label=stop polyA_site 1888..1901 /label=polya misc_feature 1861..1901 /label=BBa_B0012 terminator 1861..1901 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 721 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 781 ggcctttctg cgtttatata ctagagacct gtacgatcct acaggtgctt atgttaagta 841 attgtattcc cagcgataca atagtgtgac aaaaatccaa tttattagaa tcaaatgtca 901 atccattacc gttttaatga tatataacac gcaaaacttg cgacaaacaa taggtaatac 961 tagagaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa 1021 ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga 1081 ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta 1141 aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg 1201 ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta 1261 attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag 1321 cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct 1381 tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac 1441 atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa 1501 atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt 1561 gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta 1621 ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa 1681 gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac 1741 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 1801 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1861 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //