LOCUS BBa_S01998 5393 bp DNA linear 23-Dec-2024 DEFINITION Intermediate part from assembly 240 ACCESSION BBa_S01998 VERSION BBa_S01998.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..741 /label=BBa_C0040 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 misc_feature 912..930 /label=TetR 1 misc_feature 912..965 /label=BBa_R0040 promoter 931..936 /label=-35 misc_feature 937..955 /label=TetR 2 promoter 954..959 /label=-10 promoter 912..965 /label=BBa_R0040 misc_feature 978..981 /label=conserved RBS 974..985 /label=BBa_B0034 misc_feature 2081..2119 /label=LVA CDS 992..2119 /label=lacI-LVA misc_feature 992..2119 /label=BBa_C0012 misc_feature 2120..2144 /label=Help:Barcodes CDS 992..2119 /label=BBa_C0012 stem_loop 2164..2207 /label=stem_loop misc_feature 2153..2232 /label=BBa_B0010 terminator 2153..2232 /label=BBa_B0010 stem_loop 2248..2267 /label=T7 TE misc_feature 2274..2274 /label=stop polyA_site 2268..2281 /label=polya misc_feature 2241..2281 /label=BBa_B0012 terminator 2241..2281 /label=BBa_B0012 CDS 2290..2377 /label=end of LacI coding region (inactive) misc_feature 2290..2489 /label=BBa_R0010 misc_feature 2378..2415 /label=CAP binding site promoter 2426..2431 /label=-35 promoter 2450..2455 /label=-10 misc_feature 2455..2489 /label=LacI binding site misc_feature 2462..2462 /label=start promoter 2290..2489 /label=BBa_R0010 misc_feature 2502..2505 /label=conserved RBS 2498..2509 /label=BBa_B0034 CDS 2519..3226 /label=cI lambda misc_feature 3227..3259 /label=LVA misc_feature 3266..3290 /label=Help:Barcodes CDS 2516..3265 /label=BBa_C0051 stem_loop 3310..3353 /label=stem_loop misc_feature 3299..3378 /label=BBa_B0010 terminator 3299..3378 /label=BBa_B0010 stem_loop 3394..3413 /label=T7 TE misc_feature 3420..3420 /label=stop polyA_site 3414..3427 /label=polya misc_feature 3387..3427 /label=BBa_B0012 terminator 3387..3427 /label=BBa_B0012 promoter 3473..3478 /label=-10 promoter 3450..3455 /label=-35 misc_feature 3460..3476 /label=OR1 misc_feature 3436..3452 /label=OR2 misc_feature 3436..3484 /label=BBa_R0051 promoter 3436..3484 /label=BBa_R0051 misc_feature 3497..3500 /label=conserved RBS 3493..3504 /label=BBa_B0034 misc_feature 4090..4121 /label=LVA CDS 3511..4089 /label=luxI misc_feature 3511..4128 /label=BBa_C0061 misc_feature 4129..4153 /label=Help:Barcodes CDS 3511..4128 /label=BBa_C0061 stem_loop 4173..4216 /label=stem_loop misc_feature 4162..4241 /label=BBa_B0010 terminator 4162..4241 /label=BBa_B0010 stem_loop 4257..4276 /label=T7 TE misc_feature 4283..4283 /label=stop polyA_site 4277..4290 /label=polya misc_feature 4250..4290 /label=BBa_B0012 terminator 4250..4290 /label=BBa_B0012 misc_feature 4299..4318 /label=LuxR/HSL promoter 4413..4420 /label=-10 promoter 4387..4392 /label=-35 misc_feature 4426..4426 /label=start misc_feature 4428..4447 /label=Putative LuxR/HSL misc_feature 4299..4449 /label=BBa_R0063 promoter 4299..4449 /label=BBa_R0063 misc_feature 4462..4465 /label=conserved RBS 4458..4469 /label=BBa_B0034 misc_feature 4476..4477 /label=prefix misc_feature 4649..4649 /label=T misc_feature 4967..4967 /label=A CDS 4476..5225 /label=luxR misc_feature 4476..5231 /label=BBa_C0062 misc_feature 5232..5256 /label=Help:Barcodes CDS 4476..5231 /label=BBa_C0062 stem_loop 5276..5319 /label=stem_loop misc_feature 5265..5344 /label=BBa_B0010 terminator 5265..5344 /label=BBa_B0010 stem_loop 5360..5379 /label=T7 TE misc_feature 5386..5386 /label=stop polyA_site 5380..5393 /label=polya misc_feature 5353..5393 /label=BBa_B0012 terminator 5353..5393 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 961 agcactacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata 1021 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 1081 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 1141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 1201 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 1261 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 1321 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 1381 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 1441 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 1501 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 1561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 1621 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 1681 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 1741 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 1801 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 1861 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 1921 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 1981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 2041 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2281 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 2341 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 2401 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 2461 gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat actagatgag 2521 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat 2581 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg 2641 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa 2701 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc 2761 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta 2821 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt 2881 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt 2941 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc 3001 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat 3061 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt 3121 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt 3181 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga 3241 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagcc 3301 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3361 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3421 gtttatatac tagagtaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg 3481 ttgctactag agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt 3541 ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag 3601 caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac 3661 tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt 3721 ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa 3781 cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat 3841 agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga agctatatat 3901 aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag 3961 cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta 4021 ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca 4081 gtcttaaatg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 4141 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 4201 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 4261 caccttcggg tgggcctttc tgcgtttata tactagagac ctgtacgatc ctacaggtgc 4321 ttatgttaag taattgtatt cccagcgata caatagtgtg acaaaaatcc aatttattag 4381 aatcaaatgt caatccatta ccgttttaat gatatataac acgcaaaact tgcgacaaac 4441 aataggtaat actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca 4501 catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct 4561 tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc 4621 attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat 4681 attatgatga cgctaattta ataaaatatg atcctatagt agattattct aactccaatc 4741 attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg 4801 taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg 4861 ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata 4921 gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt 4981 atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag 5041 aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt 5101 gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa 5161 accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta 5221 aaaattaata acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa 5281 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 5341 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //